Chronic Intake of the Selective Serotonin Reuptake Inhibitor Fluoxetine Enhances Atherosclerosis

Martina Rami, Raquel Guillamat-Prats, Petteri Rinne, Melanie Salvermoser, Larisa Ring, Mariaelvy Bianchini, Xavier Blanchet, Remco T. A. Megens, Yvonne Doring, Barbara Walzog, Oliver Soehnlein, Christian Weber, Alexander Faussner, Sabine Steffens*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Web of Science)

Abstract

Objective Cardiovascular diseases and depression are the leading causes of disability in Western countries. Clinical data on potential cardiovascular effects of serotonin reuptake inhibitors (SSRIs), the most commonly used antidepressant drugs, are controversial. In addition to blocking serotonin reuptake transporter in the brain, SSRIs deplete the major peripheral serotonin (5-hydroxytryptamine [5-HT]) storage by inhibiting serotonin reuptake transporter-mediated uptake in platelets. In this study, we aimed to investigate the effect of chronic SSRI intake on the development of atherosclerosis. Approach and Results Treatment of apolipoprotein E-deficient mice with the SSRI fluoxetine for 2, 4, or 16 weeks increased atherosclerotic lesion formation, with most pronounced effect during early plaque development. Intravital microscopy of carotid arteries revealed enhanced myeloid cell adhesion on fluoxetine treatment. Mechanistically, we found that fluoxetine augmented vascular permeability and increased chemokine-induced integrin-binding activity of circulating leukocytes. In vitro stimulation of murine blood demonstrated that fluoxetine, but not 5-HT, could directly promote 1 and 2 integrin activation provided C-C motif chemokine ligand 5 was also present. Similar effects were observed with the SSRI escitalopram. Enhanced C-C motif chemokine ligand 5-induced integrin activation by fluoxetine was also confirmed in a human neutrophil-like cell line. In contrast to the proatherogenic properties of fluoxetine, pharmacological inhibition of the peripheral 5-HT synthesizing enzyme tryptophan hydroxylase 1 did not promote atherosclerosis, suggesting that the proatherogenic effect of fluoxetine occurs independent of peripheral 5-HT depletion. Conclusions SSRI intake may promote atherosclerosis and therefore potentially increase the risk for acute cardiovascular events by a mechanism that is independent of 5-HT depletion.
Original languageEnglish
Pages (from-to)1007-1019
Number of pages13
JournalArteriosclerosis Thrombosis and Vascular Biology
Volume38
Issue number5
DOIs
Publication statusPublished - 1 May 2018

Keywords

  • antidepressive agents
  • atherosclerosis
  • fluoxetine
  • integrins
  • serotonin
  • MYELOID CELL RECRUITMENT
  • INTEGRIN ALPHA-IIB-BETA-3
  • CARDIOVASCULAR-DISEASE
  • PERIPHERAL SEROTONIN
  • APOLIPOPROTEIN-E
  • DEPRESSION
  • MICE
  • RISK
  • ACTIVATION
  • DEFICIENT

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