Chromosome aberrations in adenomas of the colon. Proof of trisomy 7 in tumor cells by combined interphase cytogenetics and immunocytochemistry

J. Herbergs, A.P. de Bruine, P.T. Marx, M.I. Vallinga, R.W. Stockbrügger, F.C.S. Ramaekers, J.W. Arends, A.H.N. Hopman

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Abstract

Chromosome aberrations in adenomas of the colon. Proof of trisomy 7 in tumor cells by combined interphase cytogenetics and immunocytochemistry.

Herbergs J, de Bruine AP, Marx PT, Vallinga MI, Stockbrugger RW, Ramaekers FC, Arends JW, Hopman AH.

Department of Pathology, University Hospital, Maastricht, The Netherlands.

Thirty-five colon adenomas from 26 patients were analyzed with centromeric probes for chromosomes 1, 7, 17, X and Y in order to study numerical aberrations, chromosome imbalances, aneuploidy and tetraploidization. The fluorescent in situ hybridization (FISH) technique was applied to single-cell suspensions and a combination of FISH and immunocytochemistry (ICC) was employed to identify the cell type under study. Trisomy of chromosome 7 was detected in 37% of the cases. In 7 out of 13 cases this aberration was combined with abnormalities of one or 2 of the other investigated chromosomes. No correlation could be demonstrated between any of the detected chromosomal aberrations and size, localization or degree of epithelial dysplasia. With the combined FISH/ICC procedure, the abnormal cells were shown to be of epithelial rather than of stromal origin. Our data indicate that trisomy 7 is a common chromosome aberration in the epithelial component of colon adenomas
Original languageEnglish
Pages (from-to)781-785
JournalInternational Journal of Cancer
Volume57
Issue number6
DOIs
Publication statusPublished - 1 Jan 1994

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