Cellulase and Alkaline Treatment Improve Intestinal Microbial Degradation of Recalcitrant Fibers of Rapeseed Meal in Pigs

Cheng Long, Christiane Rösch, Sonja de Vries, Henk A Schols, Koen Venema*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Web of Science)

Abstract

The aim of the current study was to investigate whether degradation of rapeseed meal (RSM) by a swine gut microbiota consortium was improved by modifying RSM by treatment with cellulase (CELL), two pectinases (PECT), or alkaline (ALK) compared to untreated RSM and to assess whether microbiota composition and activity changed. The predicted relative abundances of carbohydrate digestion and absorption, glycolysis, pentose phosphate pathway, and pyruvate metabolism were significantly increased upon CELL and ALK feeding, and CELL and ALK also exhibited increased total short-chain fatty acid (SCFA) production compared to CON. Megasphaera, Prevotella, and Desulfovibrio were significantly positively correlated with SCFA production. Findings were validated in ileal cannulated pigs, which showed that CELL and ALK increased fiber degradation of RSM. In conclusion, CELL and ALK rather than PECT1 or PECT2 increased fiber degradation in RSM, and this information could guide feed additive strategies to improve efficiency and productivity in the swine industry.

Original languageEnglish
Pages (from-to)11011-11025
Number of pages15
JournalJournal of Agricultural and Food Chemistry
Volume68
Issue number39
Early online date1 Sep 2020
DOIs
Publication statusPublished - 30 Sep 2020

Keywords

  • rapeseed meal
  • pig gut microbiota
  • polysaccharides
  • carbohydrase
  • cell wall
  • WALL DEGRADING ENZYMES
  • NONSTARCH POLYSACCHARIDES
  • FIBROLYTIC ENZYMES
  • PROCESSING TECHNOLOGIES
  • NUTRIENT DIGESTIBILITY
  • CANOLA-MEAL
  • BACTERIA
  • FERMENTATION
  • DIGESTION
  • GROWTH

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