Cellulase and Alkaline Treatment Improve Intestinal Microbial Degradation of Recalcitrant Fibers of Rapeseed Meal in Pigs

Cheng Long; Christiane Rösch; Sonja de Vries; Henk A Schols; Koen Venema

doi:10.1021/acs.jafc.0c03618

Cellulase and Alkaline Treatment Improve Intestinal Microbial Degradation of Recalcitrant Fibers of Rapeseed Meal in Pigs

Cheng Long, Christiane Rösch, Sonja de Vries, Henk A Schols, Koen Venema^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The aim of the current study was to investigate whether degradation of rapeseed meal (RSM) by a swine gut microbiota consortium was improved by modifying RSM by treatment with cellulase (CELL), two pectinases (PECT), or alkaline (ALK) compared to untreated RSM and to assess whether microbiota composition and activity changed. The predicted relative abundances of carbohydrate digestion and absorption, glycolysis, pentose phosphate pathway, and pyruvate metabolism were significantly increased upon CELL and ALK feeding, and CELL and ALK also exhibited increased total short-chain fatty acid (SCFA) production compared to CON. Megasphaera, Prevotella, and Desulfovibrio were significantly positively correlated with SCFA production. Findings were validated in ileal cannulated pigs, which showed that CELL and ALK increased fiber degradation of RSM. In conclusion, CELL and ALK rather than PECT1 or PECT2 increased fiber degradation in RSM, and this information could guide feed additive strategies to improve efficiency and productivity in the swine industry.

Original language	English
Pages (from-to)	11011-11025
Number of pages	15
Journal	Journal of Agricultural and Food Chemistry
Volume	68
Issue number	39
Early online date	1 Sept 2020
DOIs	https://doi.org/10.1021/acs.jafc.0c03618
Publication status	Published - 30 Sept 2020

Keywords

rapeseed meal
pig gut microbiota
polysaccharides
carbohydrase
cell wall
WALL DEGRADING ENZYMES
NONSTARCH POLYSACCHARIDES
FIBROLYTIC ENZYMES
PROCESSING TECHNOLOGIES
NUTRIENT DIGESTIBILITY
CANOLA-MEAL
BACTERIA
FERMENTATION
DIGESTION
GROWTH

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10.1021/acs.jafc.0c03618Licence: CC BY-NC-ND

1 Erratum / corrigendum / retractions

Correction to Cellulase and Alkaline Treatment Improve Intestinal Microbial Degradation of Recalcitrant Fibers of Rapeseed Meal in Pigs
Long, C., Rösch, C., de Vries, S., Schols, H. & Venema, K., 30 Dec 2020, In: Journal of Agricultural and Food Chemistry. 68, 52, p. 15535-15535 1 p.
Research output: Contribution to journal › Erratum / corrigendum / retractions › Academic

Open Access

Cite this

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title = "Cellulase and Alkaline Treatment Improve Intestinal Microbial Degradation of Recalcitrant Fibers of Rapeseed Meal in Pigs",

abstract = "The aim of the current study was to investigate whether degradation of rapeseed meal (RSM) by a swine gut microbiota consortium was improved by modifying RSM by treatment with cellulase (CELL), two pectinases (PECT), or alkaline (ALK) compared to untreated RSM and to assess whether microbiota composition and activity changed. The predicted relative abundances of carbohydrate digestion and absorption, glycolysis, pentose phosphate pathway, and pyruvate metabolism were significantly increased upon CELL and ALK feeding, and CELL and ALK also exhibited increased total short-chain fatty acid (SCFA) production compared to CON. Megasphaera, Prevotella, and Desulfovibrio were significantly positively correlated with SCFA production. Findings were validated in ileal cannulated pigs, which showed that CELL and ALK increased fiber degradation of RSM. In conclusion, CELL and ALK rather than PECT1 or PECT2 increased fiber degradation in RSM, and this information could guide feed additive strategies to improve efficiency and productivity in the swine industry.",

keywords = "rapeseed meal, pig gut microbiota, polysaccharides, carbohydrase, cell wall, WALL DEGRADING ENZYMES, NONSTARCH POLYSACCHARIDES, FIBROLYTIC ENZYMES, PROCESSING TECHNOLOGIES, NUTRIENT DIGESTIBILITY, CANOLA-MEAL, BACTERIA, FERMENTATION, DIGESTION, GROWTH",

author = "Cheng Long and Christiane R{\"o}sch and {de Vries}, Sonja and Schols, {Henk A} and Koen Venema",

note = "Funding Information: Cheng Long received a Ph.D. scholarship from the China Scholarship Council under the Chinese Government Graduate Student Overseas Study Program (Grant No. 201603250050). Funding Information: The study was funded by the Centre for Healthy Eating & Food Innovation (HEFI) of Maastricht University–Campus Venlo and has been made possible with the support of the Dutch Province of Limburg with a Grant to HEFI, HEFI-1venk15-25. Publisher Copyright: {\textcopyright} 2020 American Chemical Society",

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doi = "10.1021/acs.jafc.0c03618",

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T1 - Cellulase and Alkaline Treatment Improve Intestinal Microbial Degradation of Recalcitrant Fibers of Rapeseed Meal in Pigs

AU - Long, Cheng

AU - Rösch, Christiane

AU - de Vries, Sonja

AU - Schols, Henk A

AU - Venema, Koen

N1 - Funding Information: Cheng Long received a Ph.D. scholarship from the China Scholarship Council under the Chinese Government Graduate Student Overseas Study Program (Grant No. 201603250050). Funding Information: The study was funded by the Centre for Healthy Eating & Food Innovation (HEFI) of Maastricht University–Campus Venlo and has been made possible with the support of the Dutch Province of Limburg with a Grant to HEFI, HEFI-1venk15-25. Publisher Copyright: © 2020 American Chemical Society

PY - 2020/9/30

Y1 - 2020/9/30

N2 - The aim of the current study was to investigate whether degradation of rapeseed meal (RSM) by a swine gut microbiota consortium was improved by modifying RSM by treatment with cellulase (CELL), two pectinases (PECT), or alkaline (ALK) compared to untreated RSM and to assess whether microbiota composition and activity changed. The predicted relative abundances of carbohydrate digestion and absorption, glycolysis, pentose phosphate pathway, and pyruvate metabolism were significantly increased upon CELL and ALK feeding, and CELL and ALK also exhibited increased total short-chain fatty acid (SCFA) production compared to CON. Megasphaera, Prevotella, and Desulfovibrio were significantly positively correlated with SCFA production. Findings were validated in ileal cannulated pigs, which showed that CELL and ALK increased fiber degradation of RSM. In conclusion, CELL and ALK rather than PECT1 or PECT2 increased fiber degradation in RSM, and this information could guide feed additive strategies to improve efficiency and productivity in the swine industry.

AB - The aim of the current study was to investigate whether degradation of rapeseed meal (RSM) by a swine gut microbiota consortium was improved by modifying RSM by treatment with cellulase (CELL), two pectinases (PECT), or alkaline (ALK) compared to untreated RSM and to assess whether microbiota composition and activity changed. The predicted relative abundances of carbohydrate digestion and absorption, glycolysis, pentose phosphate pathway, and pyruvate metabolism were significantly increased upon CELL and ALK feeding, and CELL and ALK also exhibited increased total short-chain fatty acid (SCFA) production compared to CON. Megasphaera, Prevotella, and Desulfovibrio were significantly positively correlated with SCFA production. Findings were validated in ileal cannulated pigs, which showed that CELL and ALK increased fiber degradation of RSM. In conclusion, CELL and ALK rather than PECT1 or PECT2 increased fiber degradation in RSM, and this information could guide feed additive strategies to improve efficiency and productivity in the swine industry.

KW - rapeseed meal

KW - pig gut microbiota

KW - polysaccharides

KW - carbohydrase

KW - cell wall

KW - WALL DEGRADING ENZYMES

KW - NONSTARCH POLYSACCHARIDES

KW - FIBROLYTIC ENZYMES

KW - PROCESSING TECHNOLOGIES

KW - NUTRIENT DIGESTIBILITY

KW - CANOLA-MEAL

KW - BACTERIA

KW - FERMENTATION

KW - DIGESTION

KW - GROWTH

U2 - 10.1021/acs.jafc.0c03618

DO - 10.1021/acs.jafc.0c03618

M3 - Article

C2 - 32871071

SN - 0021-8561

VL - 68

SP - 11011

EP - 11025

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 39

ER -

Cellulase and Alkaline Treatment Improve Intestinal Microbial Degradation of Recalcitrant Fibers of Rapeseed Meal in Pigs

Abstract

Keywords

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Research output

Correction to Cellulase and Alkaline Treatment Improve Intestinal Microbial Degradation of Recalcitrant Fibers of Rapeseed Meal in Pigs

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