Cardiac Troponin T: Smaller Molecules in Patients with End-Stage Renal Disease than after Onset of Acute Myocardial Infarction

Alma M. A. Mingels*, Eline P. M. Cardinaels, Natascha J. H. Broers, Anneke van Sleeuwen, Alexander S. Streng, Marja P. van Dieijen-Visser, Jeroen P. Kooman, Otto Bekers

*Corresponding author for this work

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Abstract

BACKGROUND: We have found previously that in acute myocardial infarction (AMI), cardiac troponin T (cTnT) is degraded in a time-dependent pattern. We investigated whether cTnT forms differed in patients with chronic cTnT increases, as seen with renal dysfunction, from those in the acute phase of myocardial infarction.

METHODS: We separated cTnT forms by gel filtration chromatography (GFC) in end-stage renal disease (ESRD) patients: prehemodialysis (pre-HD) and post-HD (n = 10) and 2 months follow-up (n = 6). Purified (cTnT) standards, quality control materials of the clinical cTnT immunoassay (Roche), and AMI patients' sera also were analyzed. Immunoprecipitation and Western blotting were performed with the original cTnT antibodies from the clinical assay and antibodies against the N-and C-terminal end of cTnT.

RESULTS: GFC analysis revealed the retention of purified cTnT at 27.5 mL, identical to that for cTnT in quality controls. For all ESRD patients, one cTnT peak was found at 45 mL, pre- and post-HD, and stable over time. Western blotting.illustrated that this peak corresponded to cTnT fragments

CONCLUSIONS: We found that cTnT forms in ESRD patients are small (

Original languageEnglish
Pages (from-to)683-690
Number of pages8
JournalClinical Chemistry
Volume63
Issue number3
DOIs
Publication statusPublished - Mar 2017

Keywords

  • ACUTE CORONARY SYNDROME
  • HIGH-SENSITIVITY
  • HEMODIALYSIS-PATIENTS
  • HEART-FAILURE
  • DEGRADATION
  • ASSAYS
  • ASSOCIATION
  • POPULATION
  • CHALLENGES
  • MORTALITY

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