Biofabrication strategies for 3D in vitro models and regenerative medicine

Lorenzo Moroni*, Jason A. Burdick, Christopher Highley, Sang Jin Lee, Yuya Morimoto, Shoji Takeuchi, James J. Yoo

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

7 Citations (Web of Science)
218 Downloads (Pure)

Abstract

Organs are complex systems composed of different cells, proteins and signalling molecules that are arranged in a highly ordered structure to orchestrate a myriad of functions in our body. Biofabrication strategies can be applied to engineer 3D tissue models in vitro by mimicking the structure and function of native tissue through the precise deposition and assembly of materials and cells. This approach allows the spatiotemporal control over cell-cell and cell-extracellular matrix communication and thus the recreation of tissue-like structures. In this Review, we examine biofabrication strategies for the construction of functional tissue replacements and organ models, focusing on the development of biomaterials, such as supramolecular and photosensitive materials, that can be processed using biofabrication techniques. We highlight bioprinted and bioassembled tissue models and survey biofabrication techniques for their potential to recreate complex tissue properties, such as shape, vasculature and specific functionalities. Finally, we discuss challenges, such as scalability and the foreign body response, and opportunities in the field and provide an outlook to the future of biofabrication in regenerative medicine.

Original languageEnglish
Pages (from-to)21-37
Number of pages17
JournalNature reviews materials
Volume3
Issue number5
DOIs
Publication statusPublished - May 2018

Keywords

  • ON-A-CHIP
  • FOREIGN-BODY RESPONSE
  • TISSUE-ENGINEERING APPLICATIONS
  • MATURE ALLOGENIC CHONDROCYTES
  • LIQUID INTERFACE PRODUCTION
  • PROOF-OF-CONCEPT
  • STEM-CELLS
  • LONG-TERM
  • EXTRACELLULAR MICROENVIRONMENTS
  • BIOPRINTING TECHNOLOGY

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