Biodegradation and Immunological Parameters of Polyurethane-Based Tissue Adhesive in Arterial Microvascular Anastomoses-A Long-Term In Vivo Study

Ali Modabber, Philipp Winnand, Evgeny Goloborodko, Stephan Christian Möhlhenrich, Kristian Kniha, René Tolba, Stefan Jockenhoevel, Benita Hermanns-Sachweh, Frank Hölzle, Marius Heitzer

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

In microsurgical anastomosis, non-synthetic fibrin-based adhesives have predominantly shown superior properties to synthetic cyanoacrylates, but they have hardly any clinical application. This study aims to investigate the local and systemic effects of synthetically produced biodegradable adhesive VIVO when used in microsurgical anastomosis. VIVO is used in two different anastomosis procedures in the common carotid artery in a rat model: VIVO in addition to a temporary catheter (VIVO TC) and VIVO with a custom-shaped memory nitinol stent (VIVO SM). Conventionally sutured anastomoses serve as controls (C). Tissue response is assessed by in vivo fluorescence imaging and histological examination. The systemic effects of biodegradation are measured using hematologic parameters and serum levels of transaminase activity and lactate dehydrogenase. Finally, the degree of local adhesion of the different anastomotic procedures is evaluated. Fluorescence imaging shows reduced inflammatory blood flow in the VIVO TC group. Histological analysis of the anastomosed vessels also reveals significantly more inflammation in C than in the two adhesive groups. The severity of VIVO adhesions proves acceptable, and no histotoxic effects of VIVO are detected. The data demonstrate that the synthetic tissue adhesive VIVO is a reliable and-compared to sutures-tissue-friendly adhesive for microsurgical anastomoses.

Original languageEnglish
Article number2100451
Pages (from-to)e2100451
Number of pages10
JournalMacromolecular Bioscience
Volume22
Issue number4
DOIs
Publication statusPublished - Apr 2022

Keywords

  • Adhesives
  • Anastomosis, Surgical/methods
  • Animals
  • Carotid Arteries/surgery
  • Fibrin Tissue Adhesive
  • Microsurgery/methods
  • Polyurethanes/pharmacology
  • Rats
  • Tissue Adhesives
  • HISTOACRYL GLUE
  • EFFICACY
  • tissue compatibility
  • INJURY
  • biodegradation
  • RISK
  • STENTS
  • microsurgery
  • MEDICAL ADHESIVE
  • RESTENOSIS
  • FIBRIN GLUE
  • CYANOACRYLATE
  • adhesive

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