Antigen-Specific Detection of Autoantibodies Against Myeloperoxidase (MPO) and Proteinase 3 (PR3)

J. Vanderlocht, Joyce van Beers, P. C. Limburg, Jan Damoiseaux, C. Roozendaal

Research output: Chapter in Book/Report/Conference proceedingChapterAcademic

Abstract

ANCA testing was introduced in many laboratories throughout the world when it was recognized that a significant subset of patients with small vessel vasculopathies presented with such antibodies. Many laboratories developed and introduced in-house testing methods for antigen-specific ANCA detection complementary to indirect immune fluorescence screening. Such in-house tests have proven their merit in diagnosing vasculitis and were important to identify critical steps in the development of antigen-specific assays with high sensitivity and specificity. In the meantime various commercial assays became available for antigen-specific ANCA testing. Because of the high diagnostic accuracy of such assays it can be anticipated that commercial, antigen-specific tests will completely replace in-house testing for MPO- and PR3-ANCA. Furthermore, such tests will replace the need for IIF in the diagnostic workup of AAV. In this light it can be foreseen that the knowledge that underlies the development of in-house ANCA testing will gradually disseminate over time. Therefore we describe the current antigen-specific ANCA ELISAs (direct and capture) with the intention to maintain the knowledge and the identification of the critical steps in the development of robust assays.
Original languageEnglish
Title of host publicationAutoantibodies
Subtitle of host publicationMethods and Protocols
EditorsGunnar Houen
PublisherHumana Press
Pages153-176
ISBN (Electronic)978-1-4939-8949-2
ISBN (Print)978-1-4939-8948-5
DOIs
Publication statusPublished - 2019

Publication series

SeriesMethods in Molecular Biology
ISSN1064-3745

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