Abstract
Herein, the synthesis of amylose-coated, temperature-responsive poly(N-vinylcaprolactam) (VCL)-based copolymer microgels by enzyme-catalyzed grafting-from polymerization with phosphorylase b from rabbit muscle is reported. The phosphorylase is able to recognize the oligosaccharide maltoheptaose as primer and attach glucose units from the monomer glucose-1-phosphate to it, thereby forming amylose chains while releasing inorganic phosphate. Therefore, to enable the phosphorylase-catalyzed grafting-from polymerization of glucose-1-phosphate from the PVCL-based microgels, the maltoheptaose primer is covalently attached to the microgel in the first synthesis step. This is realized by adding N-(2-aminoethyl)methacrylamide (AEMAA) as a comonomer to the PVCL microgel to integrate primary amino groups and subsequent coupling of maltoheptaonolactone. Both the PVCL/AEMAA microgel as well as the obtained microgel-maltoheptaose construct are characterized in detail by dynamic light scattering, electrophoretic mobility measurements, IR spectroscopy, and atomic force microscopy. From the microgel-maltoheptaose construct, the grafting-from polymerization of glucose-1-phosphate is performed by the addition of phosphorylase b. Atomic force microscopy images clearly demonstrate the formation of an amylose shell around the microgels. The developed amylose-coated microgels open up promising application possibilities, for example, as colloidal scavengers, since amylose helices can serve as host molecules for inclusion of hydrophobic guest molecules.
Original language | English |
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Article number | 1900144 |
Number of pages | 5 |
Journal | Macromolecular Rapid Communications |
Volume | 40 |
Issue number | 16 |
DOIs | |
Publication status | Published - Aug 2019 |
Keywords
- amylose
- enzymatic polymerization
- microgel modification
- phosphorylase
- COMPLEX-FORMATION
- TEMPERATURE
- BINDING
- PH