TY - JOUR
T1 - Amorphous magnesium phosphate-graphene oxide nano particles laden 3D-printed chitosan scaffolds with enhanced osteogenic potential and antibacterial properties
AU - Pahlevanzadeh, F.
AU - Emadi, R.
AU - Kharaziha, M.
AU - Poursamar, S. A.
AU - Nejatidanesh, F.
AU - Emadi, H.
AU - Aslani, R.
AU - Moroni, L.
AU - Setayeshmehr, M.
N1 - Funding Information:
We would like to acknowledge the support provided by the Isfahan University of Medical Sciences and the local Animal Care and Use Committee. The authors thanks Dr. Masoud Atapour (Department of Materials Engineering, Isfahan University of Technology) for contribution in editing and revising the manuscript.
Funding Information:
We would like to acknowledge the support provided by the Isfahan University of Medical Sciences and the local Animal Care and Use Committee. The authors thanks Dr. Masoud Atapour (Department of Materials Engineering, Isfahan University of Technology) for contribution in editing and revising the manuscript.
Publisher Copyright:
© 2024 Elsevier B.V.
PY - 2024/4/1
Y1 - 2024/4/1
N2 - The utilization of 3D printing technology for the fabrication of graft substitutes in bone repair holds immense promise. However, meeting the requirements for printability, bioactivity, mechanical strength, and biological properties of 3D printed structures concurrently poses a significant challenge. In this study, we introduce a novel approach by incorporating amorphous magnesium phosphate-graphene oxide (AMP-GO) into a thermo-crosslinkable chitosan/β glycerol phosphate (CS/GP) ink. We fabricated thermo-crosslinkable CS inks containing varying concentrations (10 %, 20 %, or 30 % weight) of AMP-GO. The 3D printed scaffolds incorporating 20 % AMP-GO exhibited significantly improved mechanical properties, with compressive strengths of 4.5 ± 0.06 MPa compared to 0.5 ± 0.03 MPa for CS printed scaffolds. Moreover, the CS/AMP-GO inks demonstrated enhanced antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) bacteria, attributed to the release of magnesium cations and the performance of GO. Additionally, CS/20AMP-GO ink facilitated increased adhesion, viability, proliferation, and osteogenic differentiation of mesenchymal stem cells (MSCs), as evidenced by the upregulation of ALP, COL1, and Runx2 expression, which were elevated 9.8, 6.5, and >22 times, respectively, compared to pure CS scaffolds. Considering its exceptional in vivo osteogenic potential, we anticipate that the CS/20AMP-GO ink holds great potential for 3D printing of bone grafts.
AB - The utilization of 3D printing technology for the fabrication of graft substitutes in bone repair holds immense promise. However, meeting the requirements for printability, bioactivity, mechanical strength, and biological properties of 3D printed structures concurrently poses a significant challenge. In this study, we introduce a novel approach by incorporating amorphous magnesium phosphate-graphene oxide (AMP-GO) into a thermo-crosslinkable chitosan/β glycerol phosphate (CS/GP) ink. We fabricated thermo-crosslinkable CS inks containing varying concentrations (10 %, 20 %, or 30 % weight) of AMP-GO. The 3D printed scaffolds incorporating 20 % AMP-GO exhibited significantly improved mechanical properties, with compressive strengths of 4.5 ± 0.06 MPa compared to 0.5 ± 0.03 MPa for CS printed scaffolds. Moreover, the CS/AMP-GO inks demonstrated enhanced antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) bacteria, attributed to the release of magnesium cations and the performance of GO. Additionally, CS/20AMP-GO ink facilitated increased adhesion, viability, proliferation, and osteogenic differentiation of mesenchymal stem cells (MSCs), as evidenced by the upregulation of ALP, COL1, and Runx2 expression, which were elevated 9.8, 6.5, and >22 times, respectively, compared to pure CS scaffolds. Considering its exceptional in vivo osteogenic potential, we anticipate that the CS/20AMP-GO ink holds great potential for 3D printing of bone grafts.
KW - 3D printing
KW - Amorphous magnesium phosphate
KW - Bone tissue engineering
KW - Chitosan
KW - Graphene oxide
U2 - 10.1016/j.bioadv.2024.213760
DO - 10.1016/j.bioadv.2024.213760
M3 - Article
SN - 0928-4931
VL - 158
JO - Biomaterials Advances
JF - Biomaterials Advances
M1 - 213760
ER -