Abstract
The generation of cartilage from progenitor cells for the purpose of cartilage repair is often hampered by hypertrophic differentiation of the engineered cartilaginous tissue caused by endochondral ossification. Since a healthy cartilage matrix contains high amounts of Aggrecan and COMP, we hypothesized that their supplementation in the biogel used in the generation of subperiosteal cartilage mimics the composition of the cartilage extracellular matrix environment, with beneficial properties for the engineered cartilage. Supplementation of COMP or Aggrecan was studiedin vitroduring chondrogenic differentiation of rabbit periosteum cells and periosteum-derived chondrocytes. Low melting agarose was supplemented with bovine Aggrecan, human recombinant COMP or vehicle and was injected between the bone and periosteum at the upper medial side of the tibia of New Zealand white rabbits. Generated subperiosteal cartilage tissue was analyzed for weight, GAG and DNA content and ALP activity. Key markers of different phases of endochondral ossification were measured by RT-qPCR. For thein vitroexperiments, no significant differences in chondrogenic marker expression were detected following COMP or Aggrecan supplementation, whilein vivofavorable chondrogenic marker expression was detected. Gene expression levels of hypertrophic markers as well as ALP activity were significantly decreased in the Aggrecan and COMP supplemented conditions compared to controls. The wet weight and GAG content of thein vivogenerated subperiosteal cartilage tissue was not significantly different between groups. Data demonstrate the potential of Aggrecan and COMP to favorably influence the subperiosteal microenvironment for thein vivogeneration of cartilage for the optimization of cartilage regenerative approaches.
Original language | English |
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Article number | 1036 |
Pages (from-to) | 1-11 |
Number of pages | 11 |
Journal | Frontiers in bioengineering and biotechnology |
Volume | 8 |
DOIs | |
Publication status | Published - 28 Aug 2020 |
Keywords
- chondrocyte hypertrophy
- endochondral ossification
- COMP
- aggrecan
- periosteum
- periosteal chondrogenesis
- OLIGOMERIC MATRIX PROTEIN
- ARTICULAR-CARTILAGE REPAIR
- PROGENITOR CELLS
- IN-VIVO
- SULFATED GLYCOSAMINOGLYCANS
- DIFFERENTIATION
- MUTATIONS
- HEPARIN
- BINDING
- ACTS