Aberrant DNA methylation of GATA binding protein 3 (GATA3), interleukin-4 (IL-4), and transforming growth factor-beta (TGF-beta) promoters in Behcet's disease

Yunyun Zhu, Yiguo Qiu, Hongsong Yu, Shenglan Yi, Wencheng Su, Aize Kijlstra, Peizeng Yang*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Web of Science)

Abstract

The pathogenesis of Behcet's disease (BD) remains poorly understood. The purpose of this study was to investigate whether an aberrant DNA methylation of transcriptional and inflammatory factors, including TBX21, GATA3, ROR gamma t, FOXP3, IFN-gamma, IL-4, IL-17A and TGF-beta, in CD4+ T confers risk to BD. We found that the promoter methylation level of GATA3, IL-4 and TGF-beta was significantly up-regulated in active BD patients and negatively correlated with the corresponding mRNA expression. The mRNA expression of GATA3 and TGF-beta was markedly down-regulated in active BD patients compared to healthy individuals. Treatment with corticosteroids and cyclosporine (CsA) resulted in a decrease of the methylation level of GATA3 and TGF-beta in inactive BD patients. Our results suggest that an aberrant DNA methylation of GATA3 and TGF-beta is associated with their mRNA expression and participates in the pathogenesis of BD.

Original languageEnglish
Pages (from-to)64263-64272
Number of pages10
JournalOncotarget
Volume8
Issue number38
DOIs
Publication statusPublished - 8 Sep 2017

Keywords

  • DNA methylation
  • Behcet's disease
  • CD4+T cell
  • SYSTEMIC-LUPUS-ERYTHEMATOSUS
  • KOYANAGI-HARADA SYNDROME
  • T-CELL DIFFERENTIATION
  • CYTOKINE PRODUCTION
  • GENE-EXPRESSION
  • CD4+T CELLS
  • CANCER
  • EPIGENETICS
  • PATTERN
  • HYPERMETHYLATION

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