Aberrant DNA methylation of GATA binding protein 3 (GATA3), interleukin-4 (IL-4), and transforming growth factor-beta (TGF-beta) promoters in Behcet's disease

Yunyun Zhu; Yiguo Qiu; Hongsong Yu; Shenglan Yi; Wencheng Su; Aize Kijlstra; Peizeng Yang

doi:10.18632/oncotarget.19500

Aberrant DNA methylation of GATA binding protein 3 (GATA3), interleukin-4 (IL-4), and transforming growth factor-beta (TGF-beta) promoters in Behcet's disease

Yunyun Zhu, Yiguo Qiu, Hongsong Yu, Shenglan Yi, Wencheng Su, Aize Kijlstra, Peizeng Yang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The pathogenesis of Behcet's disease (BD) remains poorly understood. The purpose of this study was to investigate whether an aberrant DNA methylation of transcriptional and inflammatory factors, including TBX21, GATA3, ROR gamma t, FOXP3, IFN-gamma, IL-4, IL-17A and TGF-beta, in CD4+ T confers risk to BD. We found that the promoter methylation level of GATA3, IL-4 and TGF-beta was significantly up-regulated in active BD patients and negatively correlated with the corresponding mRNA expression. The mRNA expression of GATA3 and TGF-beta was markedly down-regulated in active BD patients compared to healthy individuals. Treatment with corticosteroids and cyclosporine (CsA) resulted in a decrease of the methylation level of GATA3 and TGF-beta in inactive BD patients. Our results suggest that an aberrant DNA methylation of GATA3 and TGF-beta is associated with their mRNA expression and participates in the pathogenesis of BD.

Original language	English
Pages (from-to)	64263-64272
Number of pages	10
Journal	Oncotarget
Volume	8
Issue number	38
DOIs	https://doi.org/10.18632/oncotarget.19500
Publication status	Published - 8 Sept 2017

Keywords

DNA methylation
Behcet's disease
CD4+T cell
SYSTEMIC-LUPUS-ERYTHEMATOSUS
KOYANAGI-HARADA SYNDROME
T-CELL DIFFERENTIATION
CYTOKINE PRODUCTION
GENE-EXPRESSION
CD4+T CELLS
CANCER
EPIGENETICS
PATTERN
HYPERMETHYLATION

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Cite this

@article{d300ea65acde4582b7ae5cde2bdf9bee,

title = "Aberrant DNA methylation of GATA binding protein 3 (GATA3), interleukin-4 (IL-4), and transforming growth factor-beta (TGF-beta) promoters in Behcet's disease",

abstract = "The pathogenesis of Behcet's disease (BD) remains poorly understood. The purpose of this study was to investigate whether an aberrant DNA methylation of transcriptional and inflammatory factors, including TBX21, GATA3, ROR gamma t, FOXP3, IFN-gamma, IL-4, IL-17A and TGF-beta, in CD4+ T confers risk to BD. We found that the promoter methylation level of GATA3, IL-4 and TGF-beta was significantly up-regulated in active BD patients and negatively correlated with the corresponding mRNA expression. The mRNA expression of GATA3 and TGF-beta was markedly down-regulated in active BD patients compared to healthy individuals. Treatment with corticosteroids and cyclosporine (CsA) resulted in a decrease of the methylation level of GATA3 and TGF-beta in inactive BD patients. Our results suggest that an aberrant DNA methylation of GATA3 and TGF-beta is associated with their mRNA expression and participates in the pathogenesis of BD.",

keywords = "DNA methylation, Behcet's disease, CD4+T cell, SYSTEMIC-LUPUS-ERYTHEMATOSUS, KOYANAGI-HARADA SYNDROME, T-CELL DIFFERENTIATION, CYTOKINE PRODUCTION, GENE-EXPRESSION, CD4+T CELLS, CANCER, EPIGENETICS, PATTERN, HYPERMETHYLATION",

author = "Yunyun Zhu and Yiguo Qiu and Hongsong Yu and Shenglan Yi and Wencheng Su and Aize Kijlstra and Peizeng Yang",

year = "2017",

month = sep,

day = "8",

doi = "10.18632/oncotarget.19500",

language = "English",

volume = "8",

pages = "64263--64272",

journal = "Oncotarget",

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publisher = "Impact Journals LLC",

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TY - JOUR

T1 - Aberrant DNA methylation of GATA binding protein 3 (GATA3), interleukin-4 (IL-4), and transforming growth factor-beta (TGF-beta) promoters in Behcet's disease

AU - Zhu, Yunyun

AU - Qiu, Yiguo

AU - Yu, Hongsong

AU - Yi, Shenglan

AU - Su, Wencheng

AU - Kijlstra, Aize

AU - Yang, Peizeng

PY - 2017/9/8

Y1 - 2017/9/8

N2 - The pathogenesis of Behcet's disease (BD) remains poorly understood. The purpose of this study was to investigate whether an aberrant DNA methylation of transcriptional and inflammatory factors, including TBX21, GATA3, ROR gamma t, FOXP3, IFN-gamma, IL-4, IL-17A and TGF-beta, in CD4+ T confers risk to BD. We found that the promoter methylation level of GATA3, IL-4 and TGF-beta was significantly up-regulated in active BD patients and negatively correlated with the corresponding mRNA expression. The mRNA expression of GATA3 and TGF-beta was markedly down-regulated in active BD patients compared to healthy individuals. Treatment with corticosteroids and cyclosporine (CsA) resulted in a decrease of the methylation level of GATA3 and TGF-beta in inactive BD patients. Our results suggest that an aberrant DNA methylation of GATA3 and TGF-beta is associated with their mRNA expression and participates in the pathogenesis of BD.

AB - The pathogenesis of Behcet's disease (BD) remains poorly understood. The purpose of this study was to investigate whether an aberrant DNA methylation of transcriptional and inflammatory factors, including TBX21, GATA3, ROR gamma t, FOXP3, IFN-gamma, IL-4, IL-17A and TGF-beta, in CD4+ T confers risk to BD. We found that the promoter methylation level of GATA3, IL-4 and TGF-beta was significantly up-regulated in active BD patients and negatively correlated with the corresponding mRNA expression. The mRNA expression of GATA3 and TGF-beta was markedly down-regulated in active BD patients compared to healthy individuals. Treatment with corticosteroids and cyclosporine (CsA) resulted in a decrease of the methylation level of GATA3 and TGF-beta in inactive BD patients. Our results suggest that an aberrant DNA methylation of GATA3 and TGF-beta is associated with their mRNA expression and participates in the pathogenesis of BD.

KW - DNA methylation

KW - Behcet's disease

KW - CD4+T cell

KW - SYSTEMIC-LUPUS-ERYTHEMATOSUS

KW - KOYANAGI-HARADA SYNDROME

KW - T-CELL DIFFERENTIATION

KW - CYTOKINE PRODUCTION

KW - GENE-EXPRESSION

KW - CD4+T CELLS

KW - CANCER

KW - EPIGENETICS

KW - PATTERN

KW - HYPERMETHYLATION

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DO - 10.18632/oncotarget.19500

M3 - Article

C2 - 28969068

SN - 1949-2553

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