A simple, time- and cost-effective, high-throughput depletion strategy for deep plasma proteomics

A. Viode; Patrick van Zalm; K.K. Smolen; B. Fatou; D. Stevenson; M. Jha; O. Levy; J. Steen; H. Steen; IMPACC Network

doi:10.1126/sciadv.adf9717

A simple, time- and cost-effective, high-throughput depletion strategy for deep plasma proteomics

A. Viode, Patrick van Zalm, K.K. Smolen, B. Fatou, D. Stevenson, M. Jha, O. Levy, J. Steen, H. Steen^*, IMPACC Network

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

We introduce a cost-effective, robust high-throughput-compatible plasma depletion method enabling in-depth profiling of plasma that detects >1300 proteins per run with a throughput of 60 samples per day. The method has been fully validated by processing >3000 samples with no apparent batch effect at a cost for the depletion step of similar to$2.5 per sample.

Original language	English
Article number	eadf9717
Number of pages	7
Journal	Science advances
Volume	9
Issue number	13
DOIs	https://doi.org/10.1126/sciadv.adf9717
Publication status	Published - 31 Mar 2023

Keywords

RESOURCE
DATABASE
PROTEINS

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10.1126/sciadv.adf9717Licence: CC BY-NC

Cite this

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AU - Stevenson, D.

AU - Jha, M.

AU - Levy, O.

AU - Steen, J.

AU - Steen, H.

AU - IMPACC Network

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