A neuroanatomical analysis of the effects of a memory impairing dose of scopolamine in the rat brain using cytochrome c oxidase as principle marker

S. Hescham, Y. Temel, J. Casaca-Carreira, K. Arslantas, Y. Yakkioui, A. Blokland, A. Jahanshahi

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Acetylcholine plays a role in mnemonic and attentional processes, but also in locomotor and anxiety-related behavior. Receptor blockage by scopolamine can therefore induce cognitive as well as motor deficits and increase anxiety levels. Here we show that scopolamine, at a dose that has previously been found to affect learning and memory performance (0.1mg/kg i.p.), has a widespread effect on cytochrome c oxidase histochemistry in various regions of the rat brain. We found a down-regulation of cytochrome c oxidase in the nucleus basalis, in movement-related structures such as the primary motor cortex and the globus pallidus, memory-related structures such as the CA1 subfield of the hippocampus and perirhinal cortex and in the anxiety-related structures like the amygdala, which also plays a role in memory. However choline acetyltransferase levels were only affected in the CA1 subfield of the hippocampus and both, choline acetyltransferase and c-Fos expression levels were decreased in the amygdala. These findings corroborate strong cognitive behavioral effects of this drug, but also suggest possible anxiety and locomotor-related behavioral changes in subjects. Moreover, they present histochemical evidence that the effects of scopolamine are not ultimately restricted to cognitive parameters.
Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalJournal of Chemical Neuroanatomy
Volume59-60
Early online date22 Apr 2014
DOIs
Publication statusPublished - Sep 2014

Keywords

  • Scopolamine
  • Acetylcholine
  • Cytochrome c oxidase
  • Choline acetyltransferase
  • Anxiety
  • Locomotion
  • Cognition
  • HIPPOCAMPAL THETA-RHYTHM
  • MUSCARINIC ACETYLCHOLINE
  • CHOLINERGIC INNERVATION
  • DIAGONAL BAND
  • WATER MAZE
  • MEDIAL SEPTUM
  • RECEPTORS
  • NEURONS
  • INVOLVEMENT
  • PERFORMANCE

Cite this