A microfluidic droplet serial dilutor with enhanced mixing

Hoon Suk Rho; Yoonsun Yang; Leon W.M.M. Terstappen; Pamela Habibovic; Séverine Le Gac

A microfluidic droplet serial dilutor with enhanced mixing

Hoon Suk Rho^*, Yoonsun Yang, Leon W.M.M. Terstappen, Pamela Habibovic, Séverine Le Gac

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceeding › Academic › peer-review

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Abstract

We present the development of an automated microfluidic platform that is capable of creating logarithmic serial dilution by adapting pneumatically actuated microvalves. A valve-assisted droplet manipulator integrated with a peristaltic mixer allowed the accurate formation of droplets with controlled sizes and the dilution of reagent sequentially. Hence, serial dilution with flexible dilution factors was obtained in a series of nanoliter-scale droplets. We validated the mixing efficiency and droplet generating performance of the chip at various operating conditions and demonstrated an example of logarithmic serial dilution with two different dilution factors. The microfluidic droplet serial dilutor could be used as an analytical tool to evaluate various complex chemical and biochemical reactions.

Original language	English
Title of host publication	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Publisher	Chemical and Biological Microsystems Society
Pages	1114-1116
Number of pages	3
Volume	2
ISBN (Electronic)	9781510897571
Publication status	Published - 1 Jan 2018
Event	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences - Kaohsiung, Taiwan Duration: 11 Nov 2018 → 15 Nov 2018 Conference number: 22

Conference

Conference	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences
Abbreviated title	MicroTAS 2018
Country/Territory	Taiwan
City	Kaohsiung
Period	11/11/18 → 15/11/18

Keywords

Droplets
Microfluidics
Microvalves
Serial dilution

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title = "A microfluidic droplet serial dilutor with enhanced mixing",

abstract = "We present the development of an automated microfluidic platform that is capable of creating logarithmic serial dilution by adapting pneumatically actuated microvalves. A valve-assisted droplet manipulator integrated with a peristaltic mixer allowed the accurate formation of droplets with controlled sizes and the dilution of reagent sequentially. Hence, serial dilution with flexible dilution factors was obtained in a series of nanoliter-scale droplets. We validated the mixing efficiency and droplet generating performance of the chip at various operating conditions and demonstrated an example of logarithmic serial dilution with two different dilution factors. The microfluidic droplet serial dilutor could be used as an analytical tool to evaluate various complex chemical and biochemical reactions.",

keywords = "Droplets, Microfluidics, Microvalves, Serial dilution",

author = "Rho, {Hoon Suk} and Yoonsun Yang and Terstappen, {Leon W.M.M.} and Pamela Habibovic and {Le Gac}, S{\'e}verine",

note = "Publisher Copyright: Copyright {\textcopyright} (2018) by Chemical and Biological Microsystems Society. All rights reserved.; 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 ; Conference date: 11-11-2018 Through 15-11-2018",

year = "2018",

month = jan,

day = "1",

language = "English",

volume = "2",

pages = "1114--1116",

booktitle = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018",

publisher = "Chemical and Biological Microsystems Society",

address = "United States",

}

Rho, HS, Yang, Y, Terstappen, LWMM, Habibovic, P & Le Gac, S 2018, A microfluidic droplet serial dilutor with enhanced mixing. in 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. vol. 2, Chemical and Biological Microsystems Society, pp. 1114-1116, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, Kaohsiung, Taiwan, 11/11/18.

A microfluidic droplet serial dilutor with enhanced mixing. / Rho, Hoon Suk; Yang, Yoonsun; Terstappen, Leon W.M.M. et al.
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Vol. 2 Chemical and Biological Microsystems Society, 2018. p. 1114-1116.

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceeding › Academic › peer-review

TY - GEN

T1 - A microfluidic droplet serial dilutor with enhanced mixing

AU - Rho, Hoon Suk

AU - Yang, Yoonsun

AU - Terstappen, Leon W.M.M.

AU - Habibovic, Pamela

AU - Le Gac, Séverine

N1 - Conference code: 22

PY - 2018/1/1

Y1 - 2018/1/1

N2 - We present the development of an automated microfluidic platform that is capable of creating logarithmic serial dilution by adapting pneumatically actuated microvalves. A valve-assisted droplet manipulator integrated with a peristaltic mixer allowed the accurate formation of droplets with controlled sizes and the dilution of reagent sequentially. Hence, serial dilution with flexible dilution factors was obtained in a series of nanoliter-scale droplets. We validated the mixing efficiency and droplet generating performance of the chip at various operating conditions and demonstrated an example of logarithmic serial dilution with two different dilution factors. The microfluidic droplet serial dilutor could be used as an analytical tool to evaluate various complex chemical and biochemical reactions.

AB - We present the development of an automated microfluidic platform that is capable of creating logarithmic serial dilution by adapting pneumatically actuated microvalves. A valve-assisted droplet manipulator integrated with a peristaltic mixer allowed the accurate formation of droplets with controlled sizes and the dilution of reagent sequentially. Hence, serial dilution with flexible dilution factors was obtained in a series of nanoliter-scale droplets. We validated the mixing efficiency and droplet generating performance of the chip at various operating conditions and demonstrated an example of logarithmic serial dilution with two different dilution factors. The microfluidic droplet serial dilutor could be used as an analytical tool to evaluate various complex chemical and biochemical reactions.

KW - Droplets

KW - Microfluidics

KW - Microvalves

KW - Serial dilution

M3 - Conference article in proceeding

VL - 2

SP - 1114

EP - 1116

BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018

PB - Chemical and Biological Microsystems Society

T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences

Y2 - 11 November 2018 through 15 November 2018

ER -

A microfluidic droplet serial dilutor with enhanced mixing

Abstract

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Keywords

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