A Citrus and Pomegranate Complex Reduces Methylglyoxal in Healthy Elderly Subjects: Secondary Analysis of a Double-Blind Randomized Cross-Over Clinical Trial

Katarzyna Bednarska*, Izabela Fecka*, Jean L.J.M. Scheijen, Sanne Ahles, Philippe Vangrieken, Casper G. Schalkwijk

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Reactive a-dicarbonyls (a-DCs), such as methylglyoxal (MGO), glyoxal (GO), and 3-deoxyglucosone (3-DG), are potent precursors in the formation of advanced glycation end products (AGEs). In particular, MGO and MGO-derived AGEs are thought to be involved in the development of vascular complications in diabetes. Experimental studies showed that citrus and pomegranate polyphenols can scavenge a-DCs. Therefore, the aim of this study was to evaluate the effect of a citrus and pomegranate complex (CPC) on the a-DCs plasma levels in a double-blind, placebo-controlled cross-over trial, where thirty-six elderly subjects were enrolled. They received either 500 mg of Citrus sinensis peel extract and 200 mg of Punica granatum concentrate in CPC capsules or placebo capsules for 4 weeks, with a 4-week washout period in between. For the determination of a-DCs concentrations, liquid chromatography tandem mass spectrometry was used. Following four weeks of CPC supplementation, plasma levels of MGO decreased by 9.8% (-18.7 nmol/L; 95% CI: -36.7, -0.7 nmol/L; p = 0.042). Our findings suggest that CPC supplementation may represent a promising strategy for mitigating the conditions associated with MGO involvement. This study was registered on clinicaltrials.gov as NCT03781999.
Original languageEnglish
Article number13168
Number of pages14
JournalInternational Journal of Molecular Sciences
Volume24
Issue number17
DOIs
Publication statusPublished - Sept 2023

Keywords

  • 3-deoxyglucosone
  • Citrus × sinensis
  • glyoxal
  • hesperidin
  • methylglyoxal
  • Punica × granatum
  • punicalagin
  • a-dicarbonyls

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