The effects of polyphenol supplementation on adipose tissue morphology and gene expression in overweight and obese humans

Jasper Most; Ines Warnke; Mark V. Boekschoten; Johan W. E. Jocken; Philip de Groot; Angelika Friedel; Igor Bendik; Gijs H. Goossens; Ellen E. Blaak

doi:10.1080/21623945.2018.1469942

The effects of polyphenol supplementation on adipose tissue morphology and gene expression in overweight and obese humans

Jasper Most, Ines Warnke, Mark V. Boekschoten, Johan W. E. Jocken, Philip de Groot, Angelika Friedel, Igor Bendik, Gijs H. Goossens, Ellen E. Blaak^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Dietary polyphenols have beneficial effects on adipose tissue mass and function in rodents, but human studies are scarce. In a randomized, placebo-controlled study, 25 (10 women) overweight and obese humans received a combination of the polyphenols epigallocatechin-gallate and resveratrol (282mg/d, 80mg/d, respectively, EGCG+RES, n = 11) or placebo (PLA, n = 14) supplementation for 12weeks. Abdominal subcutaneous adipose tissue (SAT) biopsies were collected for assessment of adipocyte morphology and micro-array analysis. EGCG+RES had no effects on adipocyte size and distribution compared with PLA. However, we identified pathways contributing to adipogenesis, cell cycle and apoptosis were significantly downregulated by EGCG+RES versus PLA. Furthermore, EGCG+RES significantly decreased expression of pathways related to energy metabolism, oxidative stress, inflammation, and immune defense as compared with PLA. In conclusion, the SAT gene expression profile indicates a reduced cell turnover after 12-week EGCG+RES in overweight-obese subjects. It remains to be elucidated whether these alterations translate into long-term metabolic effects.

Original language	English
Pages (from-to)	190-196
Number of pages	7
Journal	Adipocyte
Volume	7
Issue number	3
DOIs	https://doi.org/10.1080/21623945.2018.1469942
Publication status	Published - 1 Jan 2018

Keywords

Adipose tissue
EGCG
Morphology
Obesity
Resveratrol
Transcriptomics
RESVERATROL SUPPLEMENTATION
INSULIN-RESISTANCE
METABOLIC DISEASE
TURNOVER
EPIGALLOCATECHIN-3-GALLATE
MECHANISMS
WEIGHT
WOMEN
TRIAL
MICE

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10.1080/21623945.2018.1469942

Cite this

@article{8a5a80db1ea04dd28fdc3d33be335496,

title = "The effects of polyphenol supplementation on adipose tissue morphology and gene expression in overweight and obese humans",

abstract = "Dietary polyphenols have beneficial effects on adipose tissue mass and function in rodents, but human studies are scarce. In a randomized, placebo-controlled study, 25 (10 women) overweight and obese humans received a combination of the polyphenols epigallocatechin-gallate and resveratrol (282mg/d, 80mg/d, respectively, EGCG+RES, n = 11) or placebo (PLA, n = 14) supplementation for 12weeks. Abdominal subcutaneous adipose tissue (SAT) biopsies were collected for assessment of adipocyte morphology and micro-array analysis. EGCG+RES had no effects on adipocyte size and distribution compared with PLA. However, we identified pathways contributing to adipogenesis, cell cycle and apoptosis were significantly downregulated by EGCG+RES versus PLA. Furthermore, EGCG+RES significantly decreased expression of pathways related to energy metabolism, oxidative stress, inflammation, and immune defense as compared with PLA. In conclusion, the SAT gene expression profile indicates a reduced cell turnover after 12-week EGCG+RES in overweight-obese subjects. It remains to be elucidated whether these alterations translate into long-term metabolic effects.",

keywords = "Adipose tissue, EGCG, Morphology, Obesity, Resveratrol, Transcriptomics, RESVERATROL SUPPLEMENTATION, INSULIN-RESISTANCE, METABOLIC DISEASE, TURNOVER, EPIGALLOCATECHIN-3-GALLATE, MECHANISMS, WEIGHT, WOMEN, TRIAL, MICE",

author = "Jasper Most and Ines Warnke and Boekschoten, {Mark V.} and Jocken, {Johan W. E.} and {de Groot}, Philip and Angelika Friedel and Igor Bendik and Goossens, {Gijs H.} and Blaak, {Ellen E.}",

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doi = "10.1080/21623945.2018.1469942",

language = "English",

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journal = "Adipocyte",

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T1 - The effects of polyphenol supplementation on adipose tissue morphology and gene expression in overweight and obese humans

AU - Most, Jasper

AU - Warnke, Ines

AU - Boekschoten, Mark V.

AU - Jocken, Johan W. E.

AU - de Groot, Philip

AU - Friedel, Angelika

AU - Bendik, Igor

AU - Goossens, Gijs H.

AU - Blaak, Ellen E.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Dietary polyphenols have beneficial effects on adipose tissue mass and function in rodents, but human studies are scarce. In a randomized, placebo-controlled study, 25 (10 women) overweight and obese humans received a combination of the polyphenols epigallocatechin-gallate and resveratrol (282mg/d, 80mg/d, respectively, EGCG+RES, n = 11) or placebo (PLA, n = 14) supplementation for 12weeks. Abdominal subcutaneous adipose tissue (SAT) biopsies were collected for assessment of adipocyte morphology and micro-array analysis. EGCG+RES had no effects on adipocyte size and distribution compared with PLA. However, we identified pathways contributing to adipogenesis, cell cycle and apoptosis were significantly downregulated by EGCG+RES versus PLA. Furthermore, EGCG+RES significantly decreased expression of pathways related to energy metabolism, oxidative stress, inflammation, and immune defense as compared with PLA. In conclusion, the SAT gene expression profile indicates a reduced cell turnover after 12-week EGCG+RES in overweight-obese subjects. It remains to be elucidated whether these alterations translate into long-term metabolic effects.

AB - Dietary polyphenols have beneficial effects on adipose tissue mass and function in rodents, but human studies are scarce. In a randomized, placebo-controlled study, 25 (10 women) overweight and obese humans received a combination of the polyphenols epigallocatechin-gallate and resveratrol (282mg/d, 80mg/d, respectively, EGCG+RES, n = 11) or placebo (PLA, n = 14) supplementation for 12weeks. Abdominal subcutaneous adipose tissue (SAT) biopsies were collected for assessment of adipocyte morphology and micro-array analysis. EGCG+RES had no effects on adipocyte size and distribution compared with PLA. However, we identified pathways contributing to adipogenesis, cell cycle and apoptosis were significantly downregulated by EGCG+RES versus PLA. Furthermore, EGCG+RES significantly decreased expression of pathways related to energy metabolism, oxidative stress, inflammation, and immune defense as compared with PLA. In conclusion, the SAT gene expression profile indicates a reduced cell turnover after 12-week EGCG+RES in overweight-obese subjects. It remains to be elucidated whether these alterations translate into long-term metabolic effects.

KW - Adipose tissue

KW - EGCG

KW - Morphology

KW - Obesity

KW - Resveratrol

KW - Transcriptomics

KW - RESVERATROL SUPPLEMENTATION

KW - INSULIN-RESISTANCE

KW - METABOLIC DISEASE

KW - TURNOVER

KW - EPIGALLOCATECHIN-3-GALLATE

KW - MECHANISMS

KW - WEIGHT

KW - WOMEN

KW - TRIAL

KW - MICE

U2 - 10.1080/21623945.2018.1469942

DO - 10.1080/21623945.2018.1469942

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EP - 196

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JF - Adipocyte

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