TY - JOUR
T1 - The effects of fetal and perinatal asphyxia on neuronal cytokine levels and ceramide metabolism in adulthood
AU - Vlassaks, Evi
AU - Gavilanes, Antonio W. D.
AU - Vles, Johan S. H.
AU - Deville, Sarah
AU - Kramer, Boris W.
AU - Strackx, Eveline
AU - Martinez-Martinez, Pilar
PY - 2013/2/15
Y1 - 2013/2/15
N2 - In a rat model of global fetal and perinatal asphyxia, we investigated if asphyxia and long-lasting brain tolerance to asphyxia (preconditioning) are mediated by modifications in inflammatory cytokines and ceramide metabolism genes in prefrontal cortex, hippocampus and caudate-putamen at the age of 8 months. Most significant changes were found in prefrontal cortex, with reduced LAG1 homolog ceramide synthase I expression after both types of asphyxia. Additionally, sphingosine kinase 1 was upregulated in those animals that experienced the combination of fetal and perinatal asphyxia (preconditioning), suggesting increased cell proliferation. While cytokine levels are normal, levels of ceramide genes were modulated both after fetal and perinatal asphyxia in the adult prefrontal cortex. Moreover, the combination of two subsequent asphyctic insults provides long-lasting neuroprotection in the prefrontal cortex probably by maintaining normal apoptosis and promoting cell proliferation. Better understanding of the effects of asphyxia on ceramide metabolism will help to understand the changes leading to brain tolerance and will open opportunities for the development of new neuroprotective therapies.
AB - In a rat model of global fetal and perinatal asphyxia, we investigated if asphyxia and long-lasting brain tolerance to asphyxia (preconditioning) are mediated by modifications in inflammatory cytokines and ceramide metabolism genes in prefrontal cortex, hippocampus and caudate-putamen at the age of 8 months. Most significant changes were found in prefrontal cortex, with reduced LAG1 homolog ceramide synthase I expression after both types of asphyxia. Additionally, sphingosine kinase 1 was upregulated in those animals that experienced the combination of fetal and perinatal asphyxia (preconditioning), suggesting increased cell proliferation. While cytokine levels are normal, levels of ceramide genes were modulated both after fetal and perinatal asphyxia in the adult prefrontal cortex. Moreover, the combination of two subsequent asphyctic insults provides long-lasting neuroprotection in the prefrontal cortex probably by maintaining normal apoptosis and promoting cell proliferation. Better understanding of the effects of asphyxia on ceramide metabolism will help to understand the changes leading to brain tolerance and will open opportunities for the development of new neuroprotective therapies.
KW - Ceramides
KW - Neuroinflammation
KW - Perinatal asphyxia
KW - Preconditioning
U2 - 10.1016/j.jneuroim.2012.09.011
DO - 10.1016/j.jneuroim.2012.09.011
M3 - Article
C2 - 23078953
SN - 0165-5728
VL - 255
SP - 97
EP - 101
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 1-2
ER -