The effect of prebiotic fortified infant formulas on microbiota composition and dynamics in early life

Klaudyna Borewicz; Maria Suarez-Diez; Christine Hechler; Roseriet Beijers; Carolina de Weerth; Ilja Arts; John Penders; Carel Thijs; Arjen Nauta; Cordula Lindner; Ellen Van Leusen; Elaine E. Vaughan; Hauke Smidt

doi:10.1038/s41598-018-38268-x

The effect of prebiotic fortified infant formulas on microbiota composition and dynamics in early life

Klaudyna Borewicz^*, Maria Suarez-Diez, Christine Hechler, Roseriet Beijers, Carolina de Weerth, Ilja Arts, John Penders, Carel Thijs, Arjen Nauta, Cordula Lindner, Ellen Van Leusen, Elaine E. Vaughan, Hauke Smidt

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Gastrointestinal (GI) microbiota composition differs between breastfed and formula-fed infants. Today's infant formulas are often fortified with prebiotics to better mimic properties of human milk with respect to its effect on GI microbiota composition and function. We used Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments to investigate the composition of faecal microbiota in 2-12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding. We compared these results with results from infants fed traditional formulas used in the Netherlands in 2002-2003, which contained no added prebiotics. We showed that today's formulas supplemented with either scGOS (0.24-0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml) had a strong bifidogenic effect as compared to traditional formulas, and they also resulted in altered patterns of microbial colonisation within the developing infant gastrointestinal tract. We identified three microbial states (or developmental stages) in the first 12 weeks of life, with a gradual transition pattern towards a bifidobacteria dominated state. In infants receiving only fortified formulas, this transition towards the bifidobacteria dominated state was accelerated, whereas in infants receiving mixed feeding the transition was delayed, as compared to exclusively breastfed infants.

Original language	English
Article number	2434
Number of pages	13
Journal	Scientific Reports
Volume	9
DOIs	https://doi.org/10.1038/s41598-018-38268-x
Publication status	Published - 21 Feb 2019

Keywords

HUMAN-MILK OLIGOSACCHARIDES
HUMAN GUT MICROBIOME
INTESTINAL MICROBIOTA
SUPPLEMENTED FORMULA
FECAL BIFIDOBACTERIA
1ST YEAR
FECES
FLORA
ATOPY

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Cite this

@article{a56e1d9d793849d6ae30067a31bd1dc5,

title = "The effect of prebiotic fortified infant formulas on microbiota composition and dynamics in early life",

abstract = "Gastrointestinal (GI) microbiota composition differs between breastfed and formula-fed infants. Today's infant formulas are often fortified with prebiotics to better mimic properties of human milk with respect to its effect on GI microbiota composition and function. We used Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments to investigate the composition of faecal microbiota in 2-12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding. We compared these results with results from infants fed traditional formulas used in the Netherlands in 2002-2003, which contained no added prebiotics. We showed that today's formulas supplemented with either scGOS (0.24-0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml) had a strong bifidogenic effect as compared to traditional formulas, and they also resulted in altered patterns of microbial colonisation within the developing infant gastrointestinal tract. We identified three microbial states (or developmental stages) in the first 12 weeks of life, with a gradual transition pattern towards a bifidobacteria dominated state. In infants receiving only fortified formulas, this transition towards the bifidobacteria dominated state was accelerated, whereas in infants receiving mixed feeding the transition was delayed, as compared to exclusively breastfed infants.",

keywords = "HUMAN-MILK OLIGOSACCHARIDES, HUMAN GUT MICROBIOME, INTESTINAL MICROBIOTA, SUPPLEMENTED FORMULA, FECAL BIFIDOBACTERIA, 1ST YEAR, FECES, FLORA, ATOPY",

author = "Klaudyna Borewicz and Maria Suarez-Diez and Christine Hechler and Roseriet Beijers and {de Weerth}, Carolina and Ilja Arts and John Penders and Carel Thijs and Arjen Nauta and Cordula Lindner and {Van Leusen}, Ellen and Vaughan, {Elaine E.} and Hauke Smidt",

note = "Funding Information: The authors thank Edoardo Saccenti (Wageningen University, Laboratory of Systems and Synthetic Biology) for helpful discussions. We thank children and parents who participated in the KOALA and BINGO studies, and students who helped collect the data. This research was performed in the public-private partnership CarboHealth coordinated by the Carbohydrate Competence Center (CCC, www.cccresearch.nl) and financed by participating partners and allowances of the TKI Agri&Food program, Ministry of Economic Affairs of the Netherlands. The collection of breast milk and faecal samples in the KOALA study was supported by grants from the Netherlands Organization for Health Research and Development (ZonMw Grant 2001-1-1260), the Dutch Asthma Fund (Grant 3.2.03.48), FrieslandCampina (the Netherlands), and Triodos Foundation (the Netherlands). CdW{\textquoteright}s work was supported by a Jacobs Foundation Advanced Research Fellowship. Publisher Copyright: {\textcopyright} 2019, The Author(s).",

year = "2019",

month = feb,

day = "21",

doi = "10.1038/s41598-018-38268-x",

language = "English",

volume = "9",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

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TY - JOUR

T1 - The effect of prebiotic fortified infant formulas on microbiota composition and dynamics in early life

AU - Borewicz, Klaudyna

AU - Suarez-Diez, Maria

AU - Hechler, Christine

AU - Beijers, Roseriet

AU - de Weerth, Carolina

AU - Arts, Ilja

AU - Penders, John

AU - Thijs, Carel

AU - Nauta, Arjen

AU - Lindner, Cordula

AU - Van Leusen, Ellen

AU - Vaughan, Elaine E.

AU - Smidt, Hauke

N1 - Funding Information: The authors thank Edoardo Saccenti (Wageningen University, Laboratory of Systems and Synthetic Biology) for helpful discussions. We thank children and parents who participated in the KOALA and BINGO studies, and students who helped collect the data. This research was performed in the public-private partnership CarboHealth coordinated by the Carbohydrate Competence Center (CCC, www.cccresearch.nl) and financed by participating partners and allowances of the TKI Agri&Food program, Ministry of Economic Affairs of the Netherlands. The collection of breast milk and faecal samples in the KOALA study was supported by grants from the Netherlands Organization for Health Research and Development (ZonMw Grant 2001-1-1260), the Dutch Asthma Fund (Grant 3.2.03.48), FrieslandCampina (the Netherlands), and Triodos Foundation (the Netherlands). CdW’s work was supported by a Jacobs Foundation Advanced Research Fellowship. Publisher Copyright: © 2019, The Author(s).

PY - 2019/2/21

Y1 - 2019/2/21

N2 - Gastrointestinal (GI) microbiota composition differs between breastfed and formula-fed infants. Today's infant formulas are often fortified with prebiotics to better mimic properties of human milk with respect to its effect on GI microbiota composition and function. We used Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments to investigate the composition of faecal microbiota in 2-12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding. We compared these results with results from infants fed traditional formulas used in the Netherlands in 2002-2003, which contained no added prebiotics. We showed that today's formulas supplemented with either scGOS (0.24-0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml) had a strong bifidogenic effect as compared to traditional formulas, and they also resulted in altered patterns of microbial colonisation within the developing infant gastrointestinal tract. We identified three microbial states (or developmental stages) in the first 12 weeks of life, with a gradual transition pattern towards a bifidobacteria dominated state. In infants receiving only fortified formulas, this transition towards the bifidobacteria dominated state was accelerated, whereas in infants receiving mixed feeding the transition was delayed, as compared to exclusively breastfed infants.

AB - Gastrointestinal (GI) microbiota composition differs between breastfed and formula-fed infants. Today's infant formulas are often fortified with prebiotics to better mimic properties of human milk with respect to its effect on GI microbiota composition and function. We used Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments to investigate the composition of faecal microbiota in 2-12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding. We compared these results with results from infants fed traditional formulas used in the Netherlands in 2002-2003, which contained no added prebiotics. We showed that today's formulas supplemented with either scGOS (0.24-0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml) had a strong bifidogenic effect as compared to traditional formulas, and they also resulted in altered patterns of microbial colonisation within the developing infant gastrointestinal tract. We identified three microbial states (or developmental stages) in the first 12 weeks of life, with a gradual transition pattern towards a bifidobacteria dominated state. In infants receiving only fortified formulas, this transition towards the bifidobacteria dominated state was accelerated, whereas in infants receiving mixed feeding the transition was delayed, as compared to exclusively breastfed infants.

KW - HUMAN-MILK OLIGOSACCHARIDES

KW - HUMAN GUT MICROBIOME

KW - INTESTINAL MICROBIOTA

KW - SUPPLEMENTED FORMULA

KW - FECAL BIFIDOBACTERIA

KW - 1ST YEAR

KW - FECES

KW - FLORA

KW - ATOPY

U2 - 10.1038/s41598-018-38268-x

DO - 10.1038/s41598-018-38268-x

M3 - Article

C2 - 30792412

SN - 2045-2322

VL - 9

JO - Scientific Reports

JF - Scientific Reports

M1 - 2434

ER -