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Scavenger receptor collectin placenta 1 is a novel receptor involved in the uptake of myelin by phagocytes

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Associated researcher

  • Bogie, J. F. J.
  • Mailleux, J.
  • Wouters, E.
  • Jorissen, W.
  • Grajchen, E.
  • Vanmol, J.
  • Wouters, K.A.M.

  • Hellings, N.
  • Van Horsen, J.
  • Vanmierlo, T.

  • Hendriks, J. J. A.

Associated organisations

Abstract

Myelin-containing macrophages and microglia are the most abundant immune cells in active multiple sclerosis (MS) lesions. Our recent transcriptomic analysis demonstrated that collectin placenta 1 (CL-P1) is one of the most potently induced genes in macrophages after uptake of myelin. CL-P1 is a type II transmembrane protein with both a collagen-like and carbohydrate recognition domain, which plays a key role in host defense. In this study we sought to determine the dynamics of CL-P1 expression on myelin-containing phagocytes and define the role that it plays in MS lesion development. We show that myelin uptake increases the cell surface expression of CL-P1 by mouse and human macrophages, but not by primary mouse microglia in vitro. In active demyelinating MS lesions, CL-P1 immunoreactivity was localized to perivascular and parenchymal myelin-laden phagocytes. Finally, we demonstrate that CL-P1 is involved in myelin internalization as knockdown of CL-P1 markedly reduced myelin uptake. Collectively, our data indicate that CL-P1 is a novel receptor involved in myelin uptake by phagocytes and likely plays a role in MS lesion development.

    Research areas

  • Journal Article
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Details

Original languageEnglish
Article number44794
Number of pages9
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 20 Mar 2017