Abstract
Introduction: Chlamydia trachomatis (chlamydia) is the most commonly diagnosed bacterial sexually transmitted infection (STI) worldwide. The advancement of molecular techniques has made chlamydia diagnostics infinitely easier. However, molecular techniques lack the information on chlamydia viability. Where in routine diagnostics the detection of chlamydia DNA or RNA might suffice, in other patient scenarios, information on the viability of chlamydia might be essential.Areas covered: In this review, the authors discuss the specific strengths and limitations of currently available methods to evaluate chlamydia viability: conventional cell culture, messenger RNA (mRNA) detection and viability-PCR (V-PCR).PubMed and Google Scholar were searched with the following terms: Chlamydia trachomatis, Treatment failure, Anal chlamydia, Microbial viability, Culture, Viability-PCR, Messenger RNA, and Molecular diagnosticsExpert commentary: Several techniques are currently available to determine chlamydia viability and thus the clinical relevance of a positive test result in clinical samples. Depending on the underlying research question, all three discussed techniques have their merits when testing for viability. However, mRNA methods show the most promise in determining the presence of a true infection, in case the chlamydia reticulate body can be specifically detected. Further research is needed to understand how to best apply viability testing in current chlamydia diagnostics.
Original language | English |
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Pages (from-to) | 739-747 |
Number of pages | 9 |
Journal | Expert Review of Molecular Diagnostics |
Volume | 18 |
Issue number | 8 |
DOIs | |
Publication status | Published - 1 Jan 2018 |
Keywords
- Chlamydia trachomatis
- viability
- viability-PCR
- messenger-RNA
- culture
- test of cure
- anorectal chlamydia
- post-treatment positivity
- molecular diagnostics
- REAL-TIME PCR
- NEISSERIA-GONORRHOEAE INFECTIONS
- ACID AMPLIFICATION TESTS
- PHARYNGEAL CHLAMYDIA
- DEVELOPMENTAL CYCLE
- ETHIDIUM MONOAZIDE
- GENITAL-INFECTION
- MULTICENTER EVALUATION
- RECTAL CHLAMYDIA
- GENE-EXPRESSION