Regulation of mitochondrial biogenesis during myogenesis

A.H. Remels; R.C. Langen; P. Schrauwen; G. Schaart; A.M. Schols; H.R. Gosker

doi:10.1016/j.mce.2009.09.029

Regulation of mitochondrial biogenesis during myogenesis

A.H. Remels^*, R.C. Langen, P. Schrauwen, G. Schaart, A.M. Schols, H.R. Gosker

^*Corresponding author for this work

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Abstract

Pathways involved in mitochondrial biogenesis associated with myogenic differentiation are poorly defined. Therefore, C(2)C(12) myoblasts were differentiated into multi-nucleated myotubes and parameters/regulators of mitochondrial biogenesis were investigated. Mitochondrial respiration, citrate synthase- and beta-hydroxyacyl-CoA dehydrogenase activity as well as protein content of complexes I, II, III and V of the mitochondrial respiratory chain increased 4-8-fold during differentiation. Additionally, an increase in the ratio of myosin heavy chain (MyHC) slow vs MyHC fast protein content was observed. PPAR transcriptional activity and transcript levels of PPAR-alpha, the PPAR co-activator PGC-1alpha, mitochondrial transcription factor A and nuclear respiratory factor 1 increased during differentiation while expression levels of PPAR-gamma decreased. In conclusion, expression and activity levels of genes known for their regulatory role in skeletal muscle oxidative capabilities parallel the increase in oxidative parameters during the myogenic program. In particular, PGC-1alpha and PPAR-alpha may be involved in the regulation of mitochondrial biogenesis during myogenesis.

Original language	English
Pages (from-to)	113-120
Number of pages	8
Journal	Molecular and Cellular Endocrinology
Volume	315
Issue number	1-2
DOIs	https://doi.org/10.1016/j.mce.2009.09.029
Publication status	Published - 5 Feb 2010

Keywords

Peroxisome proliferator-activated receptors
Myogenesis
Mitochondrial transcription factor A
Nuclear respiratory factor 1
Skeletal muscle
ACTIVATED RECEPTOR-DELTA
OBSTRUCTIVE PULMONARY-DISEASE
NUCLEAR RESPIRATORY FACTORS
SKELETAL-MUSCLE PLASTICITY
PPAR-GAMMA
MYOBLAST DIFFERENTIATION
ENERGY-METABOLISM
EXPRESSION
EXERCISE
PGC-1-ALPHA

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10.1016/j.mce.2009.09.029

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Cite this

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title = "Regulation of mitochondrial biogenesis during myogenesis",

abstract = "Pathways involved in mitochondrial biogenesis associated with myogenic differentiation are poorly defined. Therefore, C(2)C(12) myoblasts were differentiated into multi-nucleated myotubes and parameters/regulators of mitochondrial biogenesis were investigated. Mitochondrial respiration, citrate synthase- and beta-hydroxyacyl-CoA dehydrogenase activity as well as protein content of complexes I, II, III and V of the mitochondrial respiratory chain increased 4-8-fold during differentiation. Additionally, an increase in the ratio of myosin heavy chain (MyHC) slow vs MyHC fast protein content was observed. PPAR transcriptional activity and transcript levels of PPAR-alpha, the PPAR co-activator PGC-1alpha, mitochondrial transcription factor A and nuclear respiratory factor 1 increased during differentiation while expression levels of PPAR-gamma decreased. In conclusion, expression and activity levels of genes known for their regulatory role in skeletal muscle oxidative capabilities parallel the increase in oxidative parameters during the myogenic program. In particular, PGC-1alpha and PPAR-alpha may be involved in the regulation of mitochondrial biogenesis during myogenesis.",

keywords = "Peroxisome proliferator-activated receptors, Myogenesis, Mitochondrial transcription factor A, Nuclear respiratory factor 1, Skeletal muscle, ACTIVATED RECEPTOR-DELTA, OBSTRUCTIVE PULMONARY-DISEASE, NUCLEAR RESPIRATORY FACTORS, SKELETAL-MUSCLE PLASTICITY, PPAR-GAMMA, MYOBLAST DIFFERENTIATION, ENERGY-METABOLISM, EXPRESSION, EXERCISE, PGC-1-ALPHA",

author = "A.H. Remels and R.C. Langen and P. Schrauwen and G. Schaart and A.M. Schols and H.R. Gosker",

year = "2010",

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doi = "10.1016/j.mce.2009.09.029",

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T1 - Regulation of mitochondrial biogenesis during myogenesis

AU - Remels, A.H.

AU - Langen, R.C.

AU - Schrauwen, P.

AU - Schaart, G.

AU - Schols, A.M.

AU - Gosker, H.R.

PY - 2010/2/5

Y1 - 2010/2/5

N2 - Pathways involved in mitochondrial biogenesis associated with myogenic differentiation are poorly defined. Therefore, C(2)C(12) myoblasts were differentiated into multi-nucleated myotubes and parameters/regulators of mitochondrial biogenesis were investigated. Mitochondrial respiration, citrate synthase- and beta-hydroxyacyl-CoA dehydrogenase activity as well as protein content of complexes I, II, III and V of the mitochondrial respiratory chain increased 4-8-fold during differentiation. Additionally, an increase in the ratio of myosin heavy chain (MyHC) slow vs MyHC fast protein content was observed. PPAR transcriptional activity and transcript levels of PPAR-alpha, the PPAR co-activator PGC-1alpha, mitochondrial transcription factor A and nuclear respiratory factor 1 increased during differentiation while expression levels of PPAR-gamma decreased. In conclusion, expression and activity levels of genes known for their regulatory role in skeletal muscle oxidative capabilities parallel the increase in oxidative parameters during the myogenic program. In particular, PGC-1alpha and PPAR-alpha may be involved in the regulation of mitochondrial biogenesis during myogenesis.

AB - Pathways involved in mitochondrial biogenesis associated with myogenic differentiation are poorly defined. Therefore, C(2)C(12) myoblasts were differentiated into multi-nucleated myotubes and parameters/regulators of mitochondrial biogenesis were investigated. Mitochondrial respiration, citrate synthase- and beta-hydroxyacyl-CoA dehydrogenase activity as well as protein content of complexes I, II, III and V of the mitochondrial respiratory chain increased 4-8-fold during differentiation. Additionally, an increase in the ratio of myosin heavy chain (MyHC) slow vs MyHC fast protein content was observed. PPAR transcriptional activity and transcript levels of PPAR-alpha, the PPAR co-activator PGC-1alpha, mitochondrial transcription factor A and nuclear respiratory factor 1 increased during differentiation while expression levels of PPAR-gamma decreased. In conclusion, expression and activity levels of genes known for their regulatory role in skeletal muscle oxidative capabilities parallel the increase in oxidative parameters during the myogenic program. In particular, PGC-1alpha and PPAR-alpha may be involved in the regulation of mitochondrial biogenesis during myogenesis.

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KW - Nuclear respiratory factor 1

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KW - NUCLEAR RESPIRATORY FACTORS

KW - SKELETAL-MUSCLE PLASTICITY

KW - PPAR-GAMMA

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KW - ENERGY-METABOLISM

KW - EXPRESSION

KW - EXERCISE

KW - PGC-1-ALPHA

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