PulmoStent: In Vitro to In Vivo Evaluation of a Tissue Engineered Endobronchial Stent

Anja Lena Thiebes; Nicola Kelly; Caoimhe A Sweeney; Donnacha J McGrath; Johanna Clauser; Kathrin Kurtenbach; Valentine N. Gesche; Weiluan Chen; Robbert Jan Kok; Ulrich Steinseifer; Mark Bruzzi; Barry J O'Brien; Peter E McHugh; Stefan Jockenhoevel; Christian G Cornelissen

doi:10.1007/s10439-016-1737-9

PulmoStent: In Vitro to In Vivo Evaluation of a Tissue Engineered Endobronchial Stent

Anja Lena Thiebes, Nicola Kelly, Caoimhe A Sweeney, Donnacha J McGrath, Johanna Clauser, Kathrin Kurtenbach, Valentine N. Gesche, Weiluan Chen, Robbert Jan Kok, Ulrich Steinseifer, Mark Bruzzi, Barry J O'Brien, Peter E McHugh, Stefan Jockenhoevel^*, Christian G Cornelissen

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Currently, there is no optimal treatment available for end stage tumour patients with airway stenosis. The PulmoStent concept aims on overcoming current hurdles in airway stenting by combining a nitinol stent with a nutrient-permeable membrane, which prevents tumour ingrowth. Respiratory epithelial cells can be seeded onto the cover to restore mucociliary clearance. In this study, a novel hand-braided dog bone stent was developed, covered with a polycarbonate urethane nonwoven and mechanically tested. Design and manufacturing of stent and cover were improved in an iterative process according to predefined requirements for permeability and mechanical properties and finally tested in a proof of concept animal study in sheep for up to 24 weeks. In each animal two stents were implanted, one of which was cell-seeded by endoscopic spraying in situ. We demonstrated the suitability of this membrane for our concept by glucose transport testing and in vitro culture of respiratory epithelial cells. In the animal study, no migration occurred in any of the twelve stents. There was only mild granulation tissue formation and tissue reaction; no severe mucus plugging was observed. Thus, the PulmoStent concept might be a step forward for palliative treatment of airway stenosis with a biohybrid stent device.

Original language	English
Pages (from-to)	873-883
Number of pages	11
Journal	Annals of Biomedical Engineering
Volume	45
Issue number	4
DOIs	https://doi.org/10.1007/s10439-016-1737-9
Publication status	Published - Apr 2017

Keywords

Journal Article

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10.1007/s10439-016-1737-9

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Thiebes, A. L., Kelly, N., Sweeney, C. A., McGrath, D. J., Clauser, J., Kurtenbach, K., Gesche, V. N., Chen, W., Kok, R. J., Steinseifer, U., Bruzzi, M., O'Brien, B. J., McHugh, P. E., Jockenhoevel, S., & Cornelissen, C. G. (2017). PulmoStent: In Vitro to In Vivo Evaluation of a Tissue Engineered Endobronchial Stent. Annals of Biomedical Engineering, 45(4), 873-883. https://doi.org/10.1007/s10439-016-1737-9

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abstract = "Currently, there is no optimal treatment available for end stage tumour patients with airway stenosis. The PulmoStent concept aims on overcoming current hurdles in airway stenting by combining a nitinol stent with a nutrient-permeable membrane, which prevents tumour ingrowth. Respiratory epithelial cells can be seeded onto the cover to restore mucociliary clearance. In this study, a novel hand-braided dog bone stent was developed, covered with a polycarbonate urethane nonwoven and mechanically tested. Design and manufacturing of stent and cover were improved in an iterative process according to predefined requirements for permeability and mechanical properties and finally tested in a proof of concept animal study in sheep for up to 24 weeks. In each animal two stents were implanted, one of which was cell-seeded by endoscopic spraying in situ. We demonstrated the suitability of this membrane for our concept by glucose transport testing and in vitro culture of respiratory epithelial cells. In the animal study, no migration occurred in any of the twelve stents. There was only mild granulation tissue formation and tissue reaction; no severe mucus plugging was observed. Thus, the PulmoStent concept might be a step forward for palliative treatment of airway stenosis with a biohybrid stent device.",

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author = "Thiebes, {Anja Lena} and Nicola Kelly and Sweeney, {Caoimhe A} and McGrath, {Donnacha J} and Johanna Clauser and Kathrin Kurtenbach and Gesche, {Valentine N.} and Weiluan Chen and Kok, {Robbert Jan} and Ulrich Steinseifer and Mark Bruzzi and O'Brien, {Barry J} and McHugh, {Peter E} and Stefan Jockenhoevel and Cornelissen, {Christian G}",

year = "2017",

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doi = "10.1007/s10439-016-1737-9",

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Thiebes, AL, Kelly, N, Sweeney, CA, McGrath, DJ, Clauser, J, Kurtenbach, K, Gesche, VN, Chen, W, Kok, RJ, Steinseifer, U, Bruzzi, M, O'Brien, BJ, McHugh, PE, Jockenhoevel, S & Cornelissen, CG 2017, 'PulmoStent: In Vitro to In Vivo Evaluation of a Tissue Engineered Endobronchial Stent', Annals of Biomedical Engineering, vol. 45, no. 4, pp. 873-883. https://doi.org/10.1007/s10439-016-1737-9

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T2 - In Vitro to In Vivo Evaluation of a Tissue Engineered Endobronchial Stent

AU - Thiebes, Anja Lena

AU - Kelly, Nicola

AU - Sweeney, Caoimhe A

AU - McGrath, Donnacha J

AU - Clauser, Johanna

AU - Kurtenbach, Kathrin

AU - Gesche, Valentine N.

AU - Chen, Weiluan

AU - Kok, Robbert Jan

AU - Steinseifer, Ulrich

AU - Bruzzi, Mark

AU - O'Brien, Barry J

AU - McHugh, Peter E

AU - Jockenhoevel, Stefan

AU - Cornelissen, Christian G

PY - 2017/4

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N2 - Currently, there is no optimal treatment available for end stage tumour patients with airway stenosis. The PulmoStent concept aims on overcoming current hurdles in airway stenting by combining a nitinol stent with a nutrient-permeable membrane, which prevents tumour ingrowth. Respiratory epithelial cells can be seeded onto the cover to restore mucociliary clearance. In this study, a novel hand-braided dog bone stent was developed, covered with a polycarbonate urethane nonwoven and mechanically tested. Design and manufacturing of stent and cover were improved in an iterative process according to predefined requirements for permeability and mechanical properties and finally tested in a proof of concept animal study in sheep for up to 24 weeks. In each animal two stents were implanted, one of which was cell-seeded by endoscopic spraying in situ. We demonstrated the suitability of this membrane for our concept by glucose transport testing and in vitro culture of respiratory epithelial cells. In the animal study, no migration occurred in any of the twelve stents. There was only mild granulation tissue formation and tissue reaction; no severe mucus plugging was observed. Thus, the PulmoStent concept might be a step forward for palliative treatment of airway stenosis with a biohybrid stent device.

AB - Currently, there is no optimal treatment available for end stage tumour patients with airway stenosis. The PulmoStent concept aims on overcoming current hurdles in airway stenting by combining a nitinol stent with a nutrient-permeable membrane, which prevents tumour ingrowth. Respiratory epithelial cells can be seeded onto the cover to restore mucociliary clearance. In this study, a novel hand-braided dog bone stent was developed, covered with a polycarbonate urethane nonwoven and mechanically tested. Design and manufacturing of stent and cover were improved in an iterative process according to predefined requirements for permeability and mechanical properties and finally tested in a proof of concept animal study in sheep for up to 24 weeks. In each animal two stents were implanted, one of which was cell-seeded by endoscopic spraying in situ. We demonstrated the suitability of this membrane for our concept by glucose transport testing and in vitro culture of respiratory epithelial cells. In the animal study, no migration occurred in any of the twelve stents. There was only mild granulation tissue formation and tissue reaction; no severe mucus plugging was observed. Thus, the PulmoStent concept might be a step forward for palliative treatment of airway stenosis with a biohybrid stent device.

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