Abstract
BACKGROUND: The suitability for omic analysis of biosamples collected in previous decades and currently stored in biobanks is unknown. OBJECTIVES: We evaluated the influence of handling and storage conditions of blood-derived biosamples on transcriptomic, epigenomic (CpG methylation), plasma metabolomic [ UPLC-ToFMS (ultra performance liquid chromatography-time-of-flight mass spectrometry)], and wide-target proteomic profiles. METHODS: We collected fresh blood samples without RNA preservative in heparin, EDTA, or citrate and held them at room temperature for = 8 hr after collection, but no effects were due to storage temperature. None of the variables examined significantly influenced the epigenomic profiles. No systematic influence of time-in-storage was observed in samples stored over a period of 13-17 years. CONCLUSIONS: Most samples currently stored in biobanks are amenable to meaningful omics analysis, provided that they satisfy collection and storage criteria defined in this study.
Original language | English |
---|---|
Pages (from-to) | 480-487 |
Journal | Environmental Health Perspectives |
Volume | 121 |
Issue number | 4 |
DOIs | |
Publication status | Published - Apr 2013 |
Keywords
- biomarkers
- epigenomics
- metabolomics
- metabonomics
- molecular epidemiology
- proteomics
- transcriptomics