Multiwell three-dimensional systems enable in vivo screening of immune reactions to biomaterials: a new strategy toward translational biomaterial research

Sarah Al-Maawi; Carlos Mota; Alica Kubesch; C. James Kirkpatrick; Lorenzo Moroni; Shahram Ghanaati

doi:10.1007/s10856-019-6263-5

Multiwell three-dimensional systems enable in vivo screening of immune reactions to biomaterials: a new strategy toward translational biomaterial research

Sarah Al-Maawi, Carlos Mota, Alica Kubesch, C. James Kirkpatrick, Lorenzo Moroni, Shahram Ghanaati^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

In vivo experiments are accompanied by ethical issues, including sacrificing a large number of animals as well as large costs. A new in vivo 3D screening system was developed to reduce the number of required animals without compromising the results. The present pilot study examined a multiwell array system in combination with three different collagen-based biomaterials (A, B and C) using subcutaneous implantation for 10 days and histological and histomorphometrical evaluations. The tissue reaction towards the device itself was dominated by mononuclear cells. However, three independent biomaterial-specific tissue reactions were observed in three chambers. The results showed a mononuclear cell-based tissue reaction in one chamber (A) and foreign body reaction by multinucleated giant cells in the other two chambers (B and C). Statistical analysis showed a significantly higher number of multinucleated giant cells in cases B and C than in case A (A vs. B; ***P

[GRAPHICS]

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Original language	English
Article number	61
Number of pages	6
Journal	Journal of Materials Science-Materials in Medicine
Volume	30
Issue number	6
DOIs	https://doi.org/10.1007/s10856-019-6263-5
Publication status	Published - Jun 2019

Keywords

MULTINUCLEATED GIANT-CELLS
IMPLANTATION BED
COLLAGEN MATRIX
PORCINE DERMIS
VASCULARIZATION
MEMBRANES

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10.1007/s10856-019-6263-5

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Cite this

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title = "Multiwell three-dimensional systems enable in vivo screening of immune reactions to biomaterials: a new strategy toward translational biomaterial research",

abstract = "In vivo experiments are accompanied by ethical issues, including sacrificing a large number of animals as well as large costs. A new in vivo 3D screening system was developed to reduce the number of required animals without compromising the results. The present pilot study examined a multiwell array system in combination with three different collagen-based biomaterials (A, B and C) using subcutaneous implantation for 10 days and histological and histomorphometrical evaluations. The tissue reaction towards the device itself was dominated by mononuclear cells. However, three independent biomaterial-specific tissue reactions were observed in three chambers. The results showed a mononuclear cell-based tissue reaction in one chamber (A) and foreign body reaction by multinucleated giant cells in the other two chambers (B and C). Statistical analysis showed a significantly higher number of multinucleated giant cells in cases B and C than in case A (A vs. B; ***P[GRAPHICS].",

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author = "Sarah Al-Maawi and Carlos Mota and Alica Kubesch and Kirkpatrick, {C. James} and Lorenzo Moroni and Shahram Ghanaati",

note = "Publisher Copyright: {\textcopyright} 2019, Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2019",

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Multiwell three-dimensional systems enable in vivo screening of immune reactions to biomaterials: a new strategy toward translational biomaterial research. / Al-Maawi, Sarah; Mota, Carlos; Kubesch, Alica et al.
In: Journal of Materials Science-Materials in Medicine, Vol. 30, No. 6, 61, 06.2019.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Multiwell three-dimensional systems enable in vivo screening of immune reactions to biomaterials

T2 - a new strategy toward translational biomaterial research

AU - Al-Maawi, Sarah

AU - Mota, Carlos

AU - Kubesch, Alica

AU - Kirkpatrick, C. James

AU - Moroni, Lorenzo

AU - Ghanaati, Shahram

PY - 2019/6

Y1 - 2019/6

N2 - In vivo experiments are accompanied by ethical issues, including sacrificing a large number of animals as well as large costs. A new in vivo 3D screening system was developed to reduce the number of required animals without compromising the results. The present pilot study examined a multiwell array system in combination with three different collagen-based biomaterials (A, B and C) using subcutaneous implantation for 10 days and histological and histomorphometrical evaluations. The tissue reaction towards the device itself was dominated by mononuclear cells. However, three independent biomaterial-specific tissue reactions were observed in three chambers. The results showed a mononuclear cell-based tissue reaction in one chamber (A) and foreign body reaction by multinucleated giant cells in the other two chambers (B and C). Statistical analysis showed a significantly higher number of multinucleated giant cells in cases B and C than in case A (A vs. B; ***P[GRAPHICS].

AB - In vivo experiments are accompanied by ethical issues, including sacrificing a large number of animals as well as large costs. A new in vivo 3D screening system was developed to reduce the number of required animals without compromising the results. The present pilot study examined a multiwell array system in combination with three different collagen-based biomaterials (A, B and C) using subcutaneous implantation for 10 days and histological and histomorphometrical evaluations. The tissue reaction towards the device itself was dominated by mononuclear cells. However, three independent biomaterial-specific tissue reactions were observed in three chambers. The results showed a mononuclear cell-based tissue reaction in one chamber (A) and foreign body reaction by multinucleated giant cells in the other two chambers (B and C). Statistical analysis showed a significantly higher number of multinucleated giant cells in cases B and C than in case A (A vs. B; ***P[GRAPHICS].

KW - MULTINUCLEATED GIANT-CELLS

KW - IMPLANTATION BED

KW - COLLAGEN MATRIX

KW - PORCINE DERMIS

KW - VASCULARIZATION

KW - MEMBRANES

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