Research output

MR and PET-CT monitoring of tissue-engineered vascular grafts in the ovine carotid artery

Research output: Contribution to journalArticleAcademicpeer-review

Associated researcher

  • Wolf, F.
  • Paefgen, V.
  • Winz, O.
  • Mertens, M.
  • Koch, S.
  • Gross-Weege, N.
  • Morgenroth, A.
  • Rix, A.
  • Schnoering, H.
  • Chalabi, K.
  • Jockenhövel, S.

  • Lammers, T.
  • Mottaghy, F.M.

  • Kiessling, F.
  • Mela, P.

Associated organisations

Abstract

The modification of biomaterials to comply with clinically employed monitoring techniques is a promising strategy to support clinical translation in regenerative medicine. Here, multimodal imaging of tissue-engineered vascular grafts (TEVG) was enabled by functionalizing the textile scaffold with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles. The resulting MR-imageable grafts (iTEVG) were monitored non-invasively throughout their whole life-cycle, from initial quality control to longitudinal functional evaluation in an ovine model for up to 8 weeks. Crucial features such as the complete embedding of the textile mesh in the developing tissue and the grafts' structural stability were assessed in vitro using 1T-, 3T- and 7T-MRI scanners. In vivo, the grafts were imaged by 3T-MRI and PET-CT. Contrary to unlabeled constructs, iTEVG could be delineated from native arteries and precisely localized by MRI. USPIO labeling neither induced calcifications, nor negatively affected their remodeling with respect to tissue-specific extracellular matrix composition and endothelialization. Functionality was confirmed by MR-angiography. 18F-FDG uptake (assessed via PET-CT) indicated only transient post-surgical inflammation. In conclusion, USPIO-labeling enables accurate localization of TEVG and opens up opportunities for multimodal imaging approaches to assess transplant acceptance and function. Thereby, it can support clinical decision-making on the need for further pharmacological or surgical interventions.

    Research areas

  • BIOREACTOR SYSTEM, HYDROGEL, IMAGING TECHNOLOGIES, IRON-OXIDE NANOPARTICLES, MAGNETIC-RESONANCE VISUALIZATION, MESH IMPLANTS, MRI, Multimodal monitoring, NEURAL STEM-CELLS, Non-invasive monitoring, PET-CT, SCAFFOLDS, TRANSPLANTATION, Tissue-engineered vascular graft, USPIO
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Details

Original languageEnglish
Article number119228
Pages (from-to)119228
Number of pages16
JournalBiomaterials
Volume216
DOIs
Publication statusPublished - Sep 2019