Abstract
Resin-based composites are used for bone repair applications and comprise resin matrix and different sized filler particles. Nanometer-sized filler particles improve composite's mechanical properties compared with micrometer-sized filler particles, but whether differences exist in the biological response to these composites is unknown. Natural bone comprises a nanocomposite structure, and nanoscale interactions with extracellular matrix components influence stem cell differentiation. Therefore we hypothesized that nanometer-sized filler particles in resin-based composites enhance osteogenic differentiation of stem cells showing a more bone cell-like response to mechanical loading compared with micrometer-sized filler particles. Pulsating fluid flow (PFF; 5 Hz, mean shear stress: 0.7 Pa; 1 h) rapidly, within 5 min, increased nitric oxide production in human adipose stem cells (hASCs) on nanocomposite, but not on micro-hybrid composite. PFF increased RUNX2 expression in hASCs on micro-hybrid composite, but not on nanocomposite after 2 h post-incubation. PFF did not affect mean cell orientation and shape index of hASCs on both composites. In conclusion, the PFF-increased nitric oxide production in hASCs on nanocomposite, and increased osteogenic differentiation of hASCs on micro-hybrid composite suggest different responses to mechanical loading of hASCs on composite with nanometer-sized and micrometer-sized filler particles. This might have important implications for bone tissue engineering. (c) 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2986-2994, 2017.
Original language | English |
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Pages (from-to) | 2986-2994 |
Number of pages | 9 |
Journal | Journal of Biomedical Materials Research Part A |
Volume | 105 |
Issue number | 11 |
DOIs | |
Publication status | Published - Nov 2017 |
Keywords
- BONE VOID FILLER
- FLUID SHEAR-STRESS
- MORPHOLOGY
- OSTEOCYTES
- OSTEOGENIC DIFFERENTIATION
- PRIMARY CILIA
- RESPONSES
- RESTORATIVE MATERIALS
- STIMULATION
- TISSUE
- adipose stem cells
- mechanoresponsiveness
- micro-hybrid composite
- nanocomposite
- pulsating fluid flow