Mass Spectrometry Imaging with Isomeric Resolution Enabled by Ozone-Induced Dissociation

Martin R. L. Paine, Berwyck L. J. Poad, Gert B. Eijkel, David L. Marshall, Stephen J. Blanksby, Ron M. A. Heeren, Shane R. Ellis*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Mass spectrometry imaging (MSI) enables the spatial distributions of molecules possessing different mass-to-charge ratios to be mapped within complex environments revealing regional changes at the molecular level. Even at high mass resolving power, however, these images often reflect the summed distribution of multiple isomeric molecules, each potentially possessing a unique distribution coinciding with distinct biological function(s) and metabolic origin. Herein, this chemical ambiguity is addressed through an innovative combination of ozone-induced dissociation reactions with MSI, enabling the differential imaging of isomeric lipid molecules directly from biological tissues. For the first time, we demonstrate both double bond- and sn-positional isomeric lipids exhibit distinct spatial locations within tissue. This MSI approach enables researchers to unravel local lipid molecular complexity based on both exact elemental composition and isomeric structure directly from tissues.

Original languageEnglish
Pages (from-to)10530-10534
Number of pages5
JournalAngewandte Chemie-International Edition
Volume57
Issue number33
DOIs
Publication statusPublished - 13 Aug 2018

Keywords

  • biochemistry
  • brain
  • isomers
  • mass spectrometry imaging
  • phospholipids
  • DOUBLE-BOND POSITION
  • NM ULTRAVIOLET PHOTODISSOCIATION
  • ELECTRON-IMPACT EXCITATION
  • STRUCTURAL-CHARACTERIZATION
  • IONIZATION
  • GLYCEROPHOSPHOLIPIDS
  • LIPIDOMICS
  • LIPIDS
  • PHOSPHOLIPIDS
  • PRESSURE

Cite this