Abstract
Prebiotic compounds aim at improving human health by targeting beneficial gut microbes. We used a dynamic in vitro model of the human digestive tract (M-SHIME (R)) to study the modulatory effects of long-chain arabinoxylans (LC-AX) towards luminal and mucosal microbiota. Two doses (3 and 6 g/L) of LC-AX were supplied to the proximal colon compartments of the M-SHIME, three times per day for 3 days. Next generation sequencing and multivariate statistical analysis revealed the distribution of key microbial genera across M-SHIME compartments. Supplementation of 6 WI. of LC-AX significantly increased the relative abundance of Bifidobacterium in both lumen (from 0.34% to 0.41%, 1.2-fold) and mucus (from 0.14% to 0.29%, 2.1-fold) compared with the control (P <0.001). Propionate (1.4-fold), as well as activity of p-xylanase, p-xylosidase and alpha-arabinofuranosidase (P <0.05) were also increased in the lumen. LC-AX may potentially benefit host health by stimulating Bifidobacterium abundance and its metabolic activity. (C) 2017 Elsevier Ltd. All rights reserved.
Original language | English |
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Pages (from-to) | 226-237 |
Number of pages | 12 |
Journal | Journal of Functional Foods |
Volume | 32 |
DOIs | |
Publication status | Published - May 2017 |
Keywords
- Prebiotics
- Mucus
- Bifidobacterium
- Microbiome
- Propionate
- In vitro
- IN-VITRO FERMENTATION
- WHEAT BRAN EXTRACT
- DYNAMIC GUT MODEL
- AKKERMANSIA-MUCINIPHILA
- HUMAN FECES
- GEN. NOV.
- OLIGOSACCHARIDES
- COMMUNITY
- PROPIONATE
- PATTERNS