Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR

L. van Dommelen, P.F.G. Wolffs*, F. van Tiel, N.H.T.M. Dukers, S. Herngreen, C.A.M.V.A. Bruggeman, C.J.P.A. Hoebe

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

We validated the use of stored samples for Chlamydia trachomatis research. C. trachomatis DNA was detected by real-time PCR in clinical (urine and self-taken vaginal swabs) and spiked samples using six different media, five different time points (up to 2 years), and four different temperature conditions. C. trachomatis was detected in all 423 samples, and no clinically relevant degradation impact was detected.

Original languageEnglish
Pages (from-to)990-992
Number of pages3
JournalJournal of Clinical Microbiology
Volume51
Issue number3
DOIs
Publication statusPublished - Mar 2013

Keywords

  • SPECIMENS
  • TRANSPORT
  • SURVIVAL
  • ASSAY

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