Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR
Research output: Contribution to journal › Article › Academic › peer-review
We validated the use of stored samples for Chlamydia trachomatis research. C. trachomatis DNA was detected by real-time PCR in clinical (urine and self-taken vaginal swabs) and spiked samples using six different media, five different time points (up to 2 years), and four different temperature conditions. C. trachomatis was detected in all 423 samples, and no clinically relevant degradation impact was detected.
- SPECIMENS, TRANSPORT, SURVIVAL, ASSAY