In vivo confocal microscopy and tear cytokine analysis in post-LASIK ectasia

Natasha Kishore Pahuja; Rohit Shetty; Rashmi Deshmukh; Anupam Sharma; Rudy M. M. A. Nuijts; Vishal Jhanji; Swaminathan Sethu; Arkasubhra Ghosh

doi:10.1136/bjophthalmol-2016-309142

In vivo confocal microscopy and tear cytokine analysis in post-LASIK ectasia

Natasha Kishore Pahuja, Rohit Shetty, Rashmi Deshmukh, Anupam Sharma, Rudy M. M. A. Nuijts, Vishal Jhanji, Swaminathan Sethu^*, Arkasubhra Ghosh

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Aim Corneal keratectasia is one of the complications associated with laser in situ keratomileusis (LASIK) that results in vision impairment. The pathogenesis of post-LASIK ectasia (PLE) remains underexplored. We report the tear cytokine profile and in vivo confocal microscopy (IVCM) findings in eyes with PLE.

Methods This retrospective study included age-matched 7 (14 eyes) post-LASIK controls (PLCs) and 6 (12 eyes) PLE subjects. Corneal topography was used to categorise the subjects into PLC and PLE groups. Ocular Surface Disease Index (OSDI) scores obtained were based on standard questionnaire and IVCM images were used to determine corneal dendritic cells density (DCD) and sub-basal nerve plexus morphology. Inflammatory cytokines/chemokines in the tears were quantified using flow cytometry based cytometric bead array.

Results Pentacam-based scores, OSDI scores and corneal DCD were significantly (p

Conclusion The current study found a significant difference in the tear film cytokine profile between normal and PLE eyes. Presence of increased corneal dendritic cells and altered tear cytokines suggests an ongoing inflammatory response in PLE.

Original language	English
Pages (from-to)	1604-1610
Number of pages	7
Journal	British Journal of Ophthalmology
Volume	101
Issue number	12
DOIs	https://doi.org/10.1136/bjophthalmol-2016-309142
Publication status	Published - Dec 2017

Keywords

SITU KERATOMILEUSIS
CORNEAL ECTASIA
RISK-FACTORS
OCULAR SURFACE
DRY EYE
KERATOCONUS
EXPRESSION
SURGERY
CELLS
MCP-1

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10.1136/bjophthalmol-2016-309142

Cite this

@article{110adc2b29bc42c5b1bde06b3ba6a4d4,

title = "In vivo confocal microscopy and tear cytokine analysis in post-LASIK ectasia",

abstract = "Aim Corneal keratectasia is one of the complications associated with laser in situ keratomileusis (LASIK) that results in vision impairment. The pathogenesis of post-LASIK ectasia (PLE) remains underexplored. We report the tear cytokine profile and in vivo confocal microscopy (IVCM) findings in eyes with PLE.Methods This retrospective study included age-matched 7 (14 eyes) post-LASIK controls (PLCs) and 6 (12 eyes) PLE subjects. Corneal topography was used to categorise the subjects into PLC and PLE groups. Ocular Surface Disease Index (OSDI) scores obtained were based on standard questionnaire and IVCM images were used to determine corneal dendritic cells density (DCD) and sub-basal nerve plexus morphology. Inflammatory cytokines/chemokines in the tears were quantified using flow cytometry based cytometric bead array.Results Pentacam-based scores, OSDI scores and corneal DCD were significantly (pConclusion The current study found a significant difference in the tear film cytokine profile between normal and PLE eyes. Presence of increased corneal dendritic cells and altered tear cytokines suggests an ongoing inflammatory response in PLE.",

keywords = "SITU KERATOMILEUSIS, CORNEAL ECTASIA, RISK-FACTORS, OCULAR SURFACE, DRY EYE, KERATOCONUS, EXPRESSION, SURGERY, CELLS, MCP-1",

author = "Pahuja, {Natasha Kishore} and Rohit Shetty and Rashmi Deshmukh and Anupam Sharma and Nuijts, {Rudy M. M. A.} and Vishal Jhanji and Swaminathan Sethu and Arkasubhra Ghosh",

year = "2017",

month = dec,

doi = "10.1136/bjophthalmol-2016-309142",

language = "English",

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journal = "British Journal of Ophthalmology",

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T1 - In vivo confocal microscopy and tear cytokine analysis in post-LASIK ectasia

AU - Pahuja, Natasha Kishore

AU - Shetty, Rohit

AU - Deshmukh, Rashmi

AU - Sharma, Anupam

AU - Nuijts, Rudy M. M. A.

AU - Jhanji, Vishal

AU - Sethu, Swaminathan

AU - Ghosh, Arkasubhra

PY - 2017/12

Y1 - 2017/12

N2 - Aim Corneal keratectasia is one of the complications associated with laser in situ keratomileusis (LASIK) that results in vision impairment. The pathogenesis of post-LASIK ectasia (PLE) remains underexplored. We report the tear cytokine profile and in vivo confocal microscopy (IVCM) findings in eyes with PLE.Methods This retrospective study included age-matched 7 (14 eyes) post-LASIK controls (PLCs) and 6 (12 eyes) PLE subjects. Corneal topography was used to categorise the subjects into PLC and PLE groups. Ocular Surface Disease Index (OSDI) scores obtained were based on standard questionnaire and IVCM images were used to determine corneal dendritic cells density (DCD) and sub-basal nerve plexus morphology. Inflammatory cytokines/chemokines in the tears were quantified using flow cytometry based cytometric bead array.Results Pentacam-based scores, OSDI scores and corneal DCD were significantly (pConclusion The current study found a significant difference in the tear film cytokine profile between normal and PLE eyes. Presence of increased corneal dendritic cells and altered tear cytokines suggests an ongoing inflammatory response in PLE.

AB - Aim Corneal keratectasia is one of the complications associated with laser in situ keratomileusis (LASIK) that results in vision impairment. The pathogenesis of post-LASIK ectasia (PLE) remains underexplored. We report the tear cytokine profile and in vivo confocal microscopy (IVCM) findings in eyes with PLE.Methods This retrospective study included age-matched 7 (14 eyes) post-LASIK controls (PLCs) and 6 (12 eyes) PLE subjects. Corneal topography was used to categorise the subjects into PLC and PLE groups. Ocular Surface Disease Index (OSDI) scores obtained were based on standard questionnaire and IVCM images were used to determine corneal dendritic cells density (DCD) and sub-basal nerve plexus morphology. Inflammatory cytokines/chemokines in the tears were quantified using flow cytometry based cytometric bead array.Results Pentacam-based scores, OSDI scores and corneal DCD were significantly (pConclusion The current study found a significant difference in the tear film cytokine profile between normal and PLE eyes. Presence of increased corneal dendritic cells and altered tear cytokines suggests an ongoing inflammatory response in PLE.

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KW - EXPRESSION

KW - SURGERY

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