Research output

Impedimetric detection of histamine in bowel fluids using synthetic receptors with pH-optimized binding characteristics

Research output: Contribution to journalArticleAcademicpeer-review

Associated researcher

Associated organisations

Abstract

Histamine is a biogenic amine that is indispensable in the efficient functioning of various physiological systems. In previous work, a molecularly imprinted polymer (MIP) based sensor platform with impedimetric read-out was presented which could rapidly and at low cost determine histamine concentrations in buffer solutions within pH 7-9.(1,2) For diagnostic applications, histamine should be detectable in a wider pH range as it mostly occurs in mildly acidic environments. To understand this pH-dependent response of the MIP sensor, we propose a statistical binding analysis model. Within this model, we predict the theoretical performance of MIP based on acrylic acid in the required pH range and verify these results experimentally by UV-vis spectroscopy, microgravimetry, and impedance spectroscopy. Using impedimetric read-out, specific and selective detection of histamine in the physiologically relevant nanomolar concentration range is possible in neutral and mildly acidic phosphate buffer. Finally, this sensor platform was used to analyze the histamine concentration of mildly acidic bowel fluid samples of several test persons. We show that this sensor provides reliable data in the relevant concentration regime, which was validated independently by enzyme-linked immuno sorbent assay (ELISA) tests.

    Research areas

  • MOLECULARLY IMPRINTED POLYMERS, MAST-CELLS, BIOMIMETIC SENSOR, RELEASE, PLASMA, MICRODIALYSIS, HYPOTHALAMUS, CHEMOSENSOR, SEROTONIN
View graph of relations

Details

Original languageEnglish
Pages (from-to)1475-1483
Number of pages9
JournalAnalytical Chemistry
Volume85
Issue number3
DOIs
Publication statusPublished - 5 Feb 2013