TY - JOUR
T1 - Imaging Intraplaque Inflammation in Carotid Atherosclerosis With F-18-Fluorocholine Positron Emission Tomography-Computed Tomography Prospective Study on Vulnerable Atheroma With Immunohistochemical Validation
AU - Vöö, Stefan
AU - Kwee, Robert M.
AU - Sluimer, Judith C.
AU - Schreuder, Floris H. B. M.
AU - Wierts, Roel
AU - Bauwens, Matthias
AU - Heeneman, Sylvia
AU - Cleutjens, Jack P. M.
AU - van Oostenbrugge, Robert J.
AU - Daemen, Jan-Willem H.
AU - Daemen, Mat J. A. P.
AU - Mottaghy, Felix M.
AU - Kooi, Eline
PY - 2016/5
Y1 - 2016/5
N2 - Background-F-18-fluorocholine (F-18-FCH) uptake is associated with cell proliferation and activity in tumor patients. We hypothesized that F-18-FCH could similarly be a valuable imaging tool to identify vulnerable plaques and associated intraplaque inflammation and atheroma cell proliferation. Methods and Results-Ten consecutive stroke patients (90% men, median age 66.5 years, range, 59.4-69.7) with ipsilateral >70% carotid artery stenosis and who underwent carotid endarterectomy were included in the study. Before carotid endarterectomy, all patients underwent positron emission tomography to assess maximum F-18-FCH uptake in ipsilateral symptomatic carotid plaques and contralateral asymptomatic carotid arteries, which was corrected for background activity, resulting in a maximum target-to-background ratio (TBRmax). Macrophage content was assessed in all carotid endarterectomy specimens as a percentage of CD68(+)-staining per whole plaque area (plaqueCD68(+)) and as a maximum CD68(+) percentage (maxCD68(+)) in the most inflamed section/plaque. Dynamic positron emission tomography imaging demonstrated that an interval of 10 minutes between F-18-FCH injection and positron emission tomography acquisition is appropriate for carotid plaque imaging. TBRmax in ipsilateral symptomatic carotid plaques correlated significantly with plaqueCD68(+) (Spearman's rho=0.648, P=0.043) and maxCD68(+) (rho=0.721, P=0.019) in the 10 corresponding carotid endarterectomy specimens. TBRmax was significantly higher (P=0.047) in ipsilateral symptomatic carotid plaques (median: 2.0; interquartile range [Q1-Q3], 1.5-2.5) compared with the contralateral asymptomatic carotid arteries (median: 1.4; Q1-Q3, 1.3-1.6). TBRmax was not significantly correlated to carotid artery stenosis (rho=0.506, P=0.135). Conclusions-In vivo uptake of F-18-FCH in human carotid atherosclerotic plaques correlated strongly with degree of macrophage infiltration and recent symptoms, thus F-18-FCH positron emission tomography is a promising tool for the evaluation of vulnerable plaques.
AB - Background-F-18-fluorocholine (F-18-FCH) uptake is associated with cell proliferation and activity in tumor patients. We hypothesized that F-18-FCH could similarly be a valuable imaging tool to identify vulnerable plaques and associated intraplaque inflammation and atheroma cell proliferation. Methods and Results-Ten consecutive stroke patients (90% men, median age 66.5 years, range, 59.4-69.7) with ipsilateral >70% carotid artery stenosis and who underwent carotid endarterectomy were included in the study. Before carotid endarterectomy, all patients underwent positron emission tomography to assess maximum F-18-FCH uptake in ipsilateral symptomatic carotid plaques and contralateral asymptomatic carotid arteries, which was corrected for background activity, resulting in a maximum target-to-background ratio (TBRmax). Macrophage content was assessed in all carotid endarterectomy specimens as a percentage of CD68(+)-staining per whole plaque area (plaqueCD68(+)) and as a maximum CD68(+) percentage (maxCD68(+)) in the most inflamed section/plaque. Dynamic positron emission tomography imaging demonstrated that an interval of 10 minutes between F-18-FCH injection and positron emission tomography acquisition is appropriate for carotid plaque imaging. TBRmax in ipsilateral symptomatic carotid plaques correlated significantly with plaqueCD68(+) (Spearman's rho=0.648, P=0.043) and maxCD68(+) (rho=0.721, P=0.019) in the 10 corresponding carotid endarterectomy specimens. TBRmax was significantly higher (P=0.047) in ipsilateral symptomatic carotid plaques (median: 2.0; interquartile range [Q1-Q3], 1.5-2.5) compared with the contralateral asymptomatic carotid arteries (median: 1.4; Q1-Q3, 1.3-1.6). TBRmax was not significantly correlated to carotid artery stenosis (rho=0.506, P=0.135). Conclusions-In vivo uptake of F-18-FCH in human carotid atherosclerotic plaques correlated strongly with degree of macrophage infiltration and recent symptoms, thus F-18-FCH positron emission tomography is a promising tool for the evaluation of vulnerable plaques.
KW - atherosclerosis
KW - carotid artery diseases
KW - endarterectomy
KW - fluorocholine
KW - inflammation
KW - macrophages
KW - positron-emission tomography
U2 - 10.1161/CIRCIMAGING.115.004467
DO - 10.1161/CIRCIMAGING.115.004467
M3 - Article
C2 - 27162131
SN - 1941-9651
VL - 9
JO - Circulation-Cardiovascular Imaging
JF - Circulation-Cardiovascular Imaging
IS - 5
M1 - e004467
ER -