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Identification of platelet function defects by multi-parameter assessment of thrombus formation

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Abstract

Assays measuring platelet aggregation (thrombus formation) at arterial shear rate mostly use collagen as only platelet-adhesive surface. Here we report a multi-surface and multi-parameter flow assay to characterize thrombus formation in whole blood from healthy subjects and patients with platelet function deficiencies. A systematic comparison is made of 52 adhesive surfaces with components activating the main platelet-adhesive receptors, and of eight output parameters reflecting distinct stages of thrombus formation. Three types of thrombus formation can be identified with a predicted hierarchy of the following receptors: glycoprotein (GP)VI, C-type lectin-like receptor-2 (CLEC-2)>GPIb>alpha6beta1, alphaIIbbeta3>alpha2beta1>CD36, alpha5beta1, alphavbeta3. Application with patient blood reveals distinct abnormalities in thrombus formation in patients with severe combined immune deficiency, Glanzmann's thrombasthenia, Hermansky-Pudlak syndrome, May-Hegglin anomaly or grey platelet syndrome. We suggest this test may be useful for the diagnosis of patients with suspected bleeding disorders or a pro-thrombotic tendency.

    Research areas

  • VON-WILLEBRAND-FACTOR, AFFINITY BINDING, COLLAGEN, ADHESION, FLOW, ACTIVATION, FIBRINOGEN, RECEPTOR, SITE, SHEAR
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Details

Original languageEnglish
Article number4257
Number of pages13
JournalNature Communications
Volume5
DOIs
Publication statusPublished - Jul 2014