Heat transfer resistance as a tool to quantify hybridization efficiency of DNA on a nanocrystalline diamond surface

P. Cornelis; T. Vandenryt; G. Wackers; E. Kellens; P. Losada-Perez; R. Thoelen; W. De Ceuninck; K. Eersels; S. Drijkoningen; K. Haenen; M. Peeters; B. van Grinsven; P. Wagner

doi:10.1016/j.diamond.2014.06.008

Heat transfer resistance as a tool to quantify hybridization efficiency of DNA on a nanocrystalline diamond surface

P. Cornelis, T. Vandenryt, G. Wackers, E. Kellens, P. Losada-Perez, R. Thoelen, W. De Ceuninck, K. Eersels^*, S. Drijkoningen, K. Haenen, M. Peeters, B. van Grinsven, P. Wagner

^*Corresponding author for this work

Maastricht Science Programme

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

In this article, we report on a label-free real-time method based on heat transfer resistivity for thermal monitoring of DNA denaturation and its potential to quantify DNA fragments with a specific sequence of interest. Probe DNA, consisting of a 36-mer fragment was covalently immobilized on a nanocrystalline diamond surface, created by chemical vapor deposition on a silicon substrate. Various concentrations of full matched 29-mer target DNA fragments were hybridized with this probe DNA. We observed that the change in heat transfer resistance upon denaturation depends on the concentration of target DNA used during the hybridization, which allowed us to determine the dose-response curve. Therefore, these results illustrate the potential of this technique to quantify the concentration of a specific DNA fragment and to quantify the hybridization efficiency to its probe. (C) 2014 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	32-36
Number of pages	5
Journal	Diamond and Related Materials
Volume	48
DOIs	https://doi.org/10.1016/j.diamond.2014.06.008
Publication status	Published - Sept 2014

Keywords

CVD diamond
Heat transfer resistance
DNA quantification
Hybridization efficiency analysis
SINGLE-NUCLEOTIDE POLYMORPHISMS
FIELD-EFFECT SENSORS
CHARGED MACROMOLECULES
THIN-FILMS
SPECTROSCOPY
ATTACHMENT
MICROSCOPY
MUTATIONS

Access to Document

10.1016/j.diamond.2014.06.008

Cite this

Cornelis, P., Vandenryt, T., Wackers, G., Kellens, E., Losada-Perez, P., Thoelen, R., De Ceuninck, W., Eersels, K., Drijkoningen, S., Haenen, K., Peeters, M., van Grinsven, B., & Wagner, P. (2014). Heat transfer resistance as a tool to quantify hybridization efficiency of DNA on a nanocrystalline diamond surface. Diamond and Related Materials, 48, 32-36. https://doi.org/10.1016/j.diamond.2014.06.008

@article{93e8915506f24a4d8ebf23f7959e5580,

title = "Heat transfer resistance as a tool to quantify hybridization efficiency of DNA on a nanocrystalline diamond surface",

abstract = "In this article, we report on a label-free real-time method based on heat transfer resistivity for thermal monitoring of DNA denaturation and its potential to quantify DNA fragments with a specific sequence of interest. Probe DNA, consisting of a 36-mer fragment was covalently immobilized on a nanocrystalline diamond surface, created by chemical vapor deposition on a silicon substrate. Various concentrations of full matched 29-mer target DNA fragments were hybridized with this probe DNA. We observed that the change in heat transfer resistance upon denaturation depends on the concentration of target DNA used during the hybridization, which allowed us to determine the dose-response curve. Therefore, these results illustrate the potential of this technique to quantify the concentration of a specific DNA fragment and to quantify the hybridization efficiency to its probe. (C) 2014 Elsevier B.V. All rights reserved.",

keywords = "CVD diamond, Heat transfer resistance, DNA quantification, Hybridization efficiency analysis, SINGLE-NUCLEOTIDE POLYMORPHISMS, FIELD-EFFECT SENSORS, CHARGED MACROMOLECULES, THIN-FILMS, SPECTROSCOPY, ATTACHMENT, MICROSCOPY, MUTATIONS",

author = "P. Cornelis and T. Vandenryt and G. Wackers and E. Kellens and P. Losada-Perez and R. Thoelen and {De Ceuninck}, W. and K. Eersels and S. Drijkoningen and K. Haenen and M. Peeters and {van Grinsven}, B. and P. Wagner",

year = "2014",

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doi = "10.1016/j.diamond.2014.06.008",

language = "English",

volume = "48",

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journal = "Diamond and Related Materials",

issn = "0925-9635",

publisher = "Elsevier Science",

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Cornelis, P, Vandenryt, T, Wackers, G, Kellens, E, Losada-Perez, P, Thoelen, R, De Ceuninck, W, Eersels, K, Drijkoningen, S, Haenen, K, Peeters, M, van Grinsven, B & Wagner, P 2014, 'Heat transfer resistance as a tool to quantify hybridization efficiency of DNA on a nanocrystalline diamond surface', Diamond and Related Materials, vol. 48, pp. 32-36. https://doi.org/10.1016/j.diamond.2014.06.008

TY - JOUR

T1 - Heat transfer resistance as a tool to quantify hybridization efficiency of DNA on a nanocrystalline diamond surface

AU - Cornelis, P.

AU - Vandenryt, T.

AU - Wackers, G.

AU - Kellens, E.

AU - Losada-Perez, P.

AU - Thoelen, R.

AU - De Ceuninck, W.

AU - Eersels, K.

AU - Drijkoningen, S.

AU - Haenen, K.

AU - Peeters, M.

AU - van Grinsven, B.

AU - Wagner, P.

PY - 2014/9

Y1 - 2014/9

N2 - In this article, we report on a label-free real-time method based on heat transfer resistivity for thermal monitoring of DNA denaturation and its potential to quantify DNA fragments with a specific sequence of interest. Probe DNA, consisting of a 36-mer fragment was covalently immobilized on a nanocrystalline diamond surface, created by chemical vapor deposition on a silicon substrate. Various concentrations of full matched 29-mer target DNA fragments were hybridized with this probe DNA. We observed that the change in heat transfer resistance upon denaturation depends on the concentration of target DNA used during the hybridization, which allowed us to determine the dose-response curve. Therefore, these results illustrate the potential of this technique to quantify the concentration of a specific DNA fragment and to quantify the hybridization efficiency to its probe. (C) 2014 Elsevier B.V. All rights reserved.

AB - In this article, we report on a label-free real-time method based on heat transfer resistivity for thermal monitoring of DNA denaturation and its potential to quantify DNA fragments with a specific sequence of interest. Probe DNA, consisting of a 36-mer fragment was covalently immobilized on a nanocrystalline diamond surface, created by chemical vapor deposition on a silicon substrate. Various concentrations of full matched 29-mer target DNA fragments were hybridized with this probe DNA. We observed that the change in heat transfer resistance upon denaturation depends on the concentration of target DNA used during the hybridization, which allowed us to determine the dose-response curve. Therefore, these results illustrate the potential of this technique to quantify the concentration of a specific DNA fragment and to quantify the hybridization efficiency to its probe. (C) 2014 Elsevier B.V. All rights reserved.

KW - CVD diamond

KW - Heat transfer resistance

KW - DNA quantification

KW - Hybridization efficiency analysis

KW - SINGLE-NUCLEOTIDE POLYMORPHISMS

KW - FIELD-EFFECT SENSORS

KW - CHARGED MACROMOLECULES

KW - THIN-FILMS

KW - SPECTROSCOPY

KW - ATTACHMENT

KW - MICROSCOPY

KW - MUTATIONS

U2 - 10.1016/j.diamond.2014.06.008

DO - 10.1016/j.diamond.2014.06.008

M3 - Article

SN - 0925-9635

VL - 48

SP - 32

EP - 36

JO - Diamond and Related Materials

JF - Diamond and Related Materials

ER -