Foetal Ureaplasma parvum bacteraemia as a function of gestation-dependent complement insufficiency: Evidence from a sheep model of pregnancy

Matthew W. Kemp; Shatha Ahmed; Michael L. Beeton; Matthew S. Payne; Masatoshi Saito; Yuichiro Miura; Haruo Usuda; Suhas G. Kallapur; Boris W. Kramer; Sarah J. Stock; Alan H. Jobe; John P. Newnham; Owen B. Spiller

doi:10.1111/aji.12599

Foetal Ureaplasma parvum bacteraemia as a function of gestation-dependent complement insufficiency: Evidence from a sheep model of pregnancy

Matthew W. Kemp, Shatha Ahmed, Michael L. Beeton, Matthew S. Payne, Masatoshi Saito, Yuichiro Miura, Haruo Usuda, Suhas G. Kallapur, Boris W. Kramer, Sarah J. Stock, Alan H. Jobe, John P. Newnham, Owen B. Spiller^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Problem: Complement is a central defence against sepsis, and increasing complement insufficiency in neonates of greater prematurity may predispose to increased sepsis. Ureaplasma spp. are the most frequently cultured bacteria from preterm blood samples.

Method of study: A sheep model of intrauterine Ureaplasma parvum infection was used to examine in vivo Ureaplasma bacteraemia at early and late gestational ages. Complement function and Ureaplasma killing assays were used to determine the correlation between complement potency and bactericidal activity of sera ex vivo.

Results: Ureaplasma was cultured from 50% of 95-day gestation lamb cord blood samples compared to 10% of 125-day gestation lambs. Bactericidal activity increased with increased gestational age, and a direct correlation between functional complement activity and bactericidal activity (R-2=.86; P

Conclusions: Ureaplasma bacteraemia in vivo was confined to early preterm lambs with low complement function, but Ureaplasma infection itself did not diminish complement levels.

Original language	English
Article number	12599
Number of pages	9
Journal	American Journal of Reproductive Immunology
Volume	77
Issue number	1
DOIs	https://doi.org/10.1111/aji.12599
Publication status	Published - Jan 2017

Keywords

complement
neonatal
preterm
sepsis
Ureaplasma
CANDIDA-ALBICANS INFECTION
ESCHERICHIA-COLI
INFLAMMATION
BIRTH
SERUM
UREALYTICUM
EXPOSURE
INFANTS
LAMB
SKIN

Access to Document

10.1111/aji.12599

Cite this

Kemp, M. W., Ahmed, S., Beeton, M. L., Payne, M. S., Saito, M., Miura, Y., Usuda, H., Kallapur, S. G., Kramer, B. W., Stock, S. J., Jobe, A. H., Newnham, J. P., & Spiller, O. B. (2017). Foetal Ureaplasma parvum bacteraemia as a function of gestation-dependent complement insufficiency: Evidence from a sheep model of pregnancy. American Journal of Reproductive Immunology, 77(1), Article 12599. https://doi.org/10.1111/aji.12599

@article{74b70eda57354e0aabbf65508de0e1e6,

title = "Foetal Ureaplasma parvum bacteraemia as a function of gestation-dependent complement insufficiency: Evidence from a sheep model of pregnancy",

abstract = "Problem: Complement is a central defence against sepsis, and increasing complement insufficiency in neonates of greater prematurity may predispose to increased sepsis. Ureaplasma spp. are the most frequently cultured bacteria from preterm blood samples.Method of study: A sheep model of intrauterine Ureaplasma parvum infection was used to examine in vivo Ureaplasma bacteraemia at early and late gestational ages. Complement function and Ureaplasma killing assays were used to determine the correlation between complement potency and bactericidal activity of sera ex vivo.Results: Ureaplasma was cultured from 50% of 95-day gestation lamb cord blood samples compared to 10% of 125-day gestation lambs. Bactericidal activity increased with increased gestational age, and a direct correlation between functional complement activity and bactericidal activity (R-2=.86; PConclusions: Ureaplasma bacteraemia in vivo was confined to early preterm lambs with low complement function, but Ureaplasma infection itself did not diminish complement levels.",

keywords = "complement, neonatal, preterm, sepsis, Ureaplasma, CANDIDA-ALBICANS INFECTION, ESCHERICHIA-COLI, INFLAMMATION, BIRTH, SERUM, UREALYTICUM, EXPOSURE, INFANTS, LAMB, SKIN",

author = "Kemp, {Matthew W.} and Shatha Ahmed and Beeton, {Michael L.} and Payne, {Matthew S.} and Masatoshi Saito and Yuichiro Miura and Haruo Usuda and Kallapur, {Suhas G.} and Kramer, {Boris W.} and Stock, {Sarah J.} and Jobe, {Alan H.} and Newnham, {John P.} and Spiller, {Owen B.}",

year = "2017",

month = jan,

doi = "10.1111/aji.12599",

language = "English",

volume = "77",

journal = "American Journal of Reproductive Immunology",

issn = "1046-7408",

publisher = "Wiley",

number = "1",

}

Kemp, MW, Ahmed, S, Beeton, ML, Payne, MS, Saito, M, Miura, Y, Usuda, H, Kallapur, SG, Kramer, BW, Stock, SJ, Jobe, AH, Newnham, JP & Spiller, OB 2017, 'Foetal Ureaplasma parvum bacteraemia as a function of gestation-dependent complement insufficiency: Evidence from a sheep model of pregnancy', American Journal of Reproductive Immunology, vol. 77, no. 1, 12599. https://doi.org/10.1111/aji.12599

TY - JOUR

T1 - Foetal Ureaplasma parvum bacteraemia as a function of gestation-dependent complement insufficiency

T2 - Evidence from a sheep model of pregnancy

AU - Kemp, Matthew W.

AU - Ahmed, Shatha

AU - Beeton, Michael L.

AU - Payne, Matthew S.

AU - Saito, Masatoshi

AU - Miura, Yuichiro

AU - Usuda, Haruo

AU - Kallapur, Suhas G.

AU - Kramer, Boris W.

AU - Stock, Sarah J.

AU - Jobe, Alan H.

AU - Newnham, John P.

AU - Spiller, Owen B.

PY - 2017/1

Y1 - 2017/1

N2 - Problem: Complement is a central defence against sepsis, and increasing complement insufficiency in neonates of greater prematurity may predispose to increased sepsis. Ureaplasma spp. are the most frequently cultured bacteria from preterm blood samples.Method of study: A sheep model of intrauterine Ureaplasma parvum infection was used to examine in vivo Ureaplasma bacteraemia at early and late gestational ages. Complement function and Ureaplasma killing assays were used to determine the correlation between complement potency and bactericidal activity of sera ex vivo.Results: Ureaplasma was cultured from 50% of 95-day gestation lamb cord blood samples compared to 10% of 125-day gestation lambs. Bactericidal activity increased with increased gestational age, and a direct correlation between functional complement activity and bactericidal activity (R-2=.86; PConclusions: Ureaplasma bacteraemia in vivo was confined to early preterm lambs with low complement function, but Ureaplasma infection itself did not diminish complement levels.

AB - Problem: Complement is a central defence against sepsis, and increasing complement insufficiency in neonates of greater prematurity may predispose to increased sepsis. Ureaplasma spp. are the most frequently cultured bacteria from preterm blood samples.Method of study: A sheep model of intrauterine Ureaplasma parvum infection was used to examine in vivo Ureaplasma bacteraemia at early and late gestational ages. Complement function and Ureaplasma killing assays were used to determine the correlation between complement potency and bactericidal activity of sera ex vivo.Results: Ureaplasma was cultured from 50% of 95-day gestation lamb cord blood samples compared to 10% of 125-day gestation lambs. Bactericidal activity increased with increased gestational age, and a direct correlation between functional complement activity and bactericidal activity (R-2=.86; PConclusions: Ureaplasma bacteraemia in vivo was confined to early preterm lambs with low complement function, but Ureaplasma infection itself did not diminish complement levels.

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KW - CANDIDA-ALBICANS INFECTION

KW - ESCHERICHIA-COLI

KW - INFLAMMATION

KW - BIRTH

KW - SERUM

KW - UREALYTICUM

KW - EXPOSURE

KW - INFANTS

KW - LAMB

KW - SKIN

U2 - 10.1111/aji.12599

DO - 10.1111/aji.12599

M3 - Article

C2 - 27862576

SN - 1046-7408

VL - 77

JO - American Journal of Reproductive Immunology

JF - American Journal of Reproductive Immunology

IS - 1

M1 - 12599

ER -