FADS1 genotype is distinguished by human subcutaneous adipose tissue fatty acids, but not inflammatory gene expression

Shannon L. Klingel; Armand Valsesia; Arne Astrup; Marie Kunesova; Wim H. M. Saris; Dominique Langin; Nathalie Viguerie; David M. Mutch

doi:10.1038/s41366-018-0169-z

FADS1 genotype is distinguished by human subcutaneous adipose tissue fatty acids, but not inflammatory gene expression

Shannon L. Klingel, Armand Valsesia, Arne Astrup, Marie Kunesova, Wim H. M. Saris, Dominique Langin, Nathalie Viguerie, David M. Mutch^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Background Single nucleotide polymorphisms (SNPs) in FADS1/FADS2 genes are associated with changes in serum and tissue polyunsaturated fatty acid (PUFA) content. PUFA regulate inflammatory signaling pathways in adipose tissue; however, the effect of SNPs in FADS1/FADS2 on adipose tissue inflammation is equivocal. The present study examined if SNPs in FADS1/FADS2 modify human subcutaneous adipose tissue (SAT) fatty acid profiles and the expression of genes associated with inflammation/immune function, lipid metabolism, and cellular differentiation.

Methods SAT fatty acids and the expression of 117 genes were measured in 174 men and women from the DiOGenes Study using gas chromatography and qRT-PCR, respectively. Associations between fatty acids, gene expression, and SNPs in FADS1/FADS2 were investigated by linear regression and multivariate analysis.

Results Four SNPs (rs174537, rs174546, rs174556, rs174601) in FADS

Conclusion This study showed that FADS1 genotype is distinguished by SAT fatty acid profiles, but not inflammatory gene expression.

Original language	English
Pages (from-to)	1539-1548
Number of pages	10
Journal	International Journal of Obesity
Volume	43
Issue number	8
DOIs	https://doi.org/10.1038/s41366-018-0169-z
Publication status	Published - Aug 2019

Keywords

DESATURASE ACTIVITY
ARACHIDONIC-ACID
DELTA-6
CLUSTER
HEALTH

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10.1038/s41366-018-0169-z

Cite this

@article{bd82c904711f4829b49cb423378a770e,

title = "FADS1 genotype is distinguished by human subcutaneous adipose tissue fatty acids, but not inflammatory gene expression",

abstract = "Background Single nucleotide polymorphisms (SNPs) in FADS1/FADS2 genes are associated with changes in serum and tissue polyunsaturated fatty acid (PUFA) content. PUFA regulate inflammatory signaling pathways in adipose tissue; however, the effect of SNPs in FADS1/FADS2 on adipose tissue inflammation is equivocal. The present study examined if SNPs in FADS1/FADS2 modify human subcutaneous adipose tissue (SAT) fatty acid profiles and the expression of genes associated with inflammation/immune function, lipid metabolism, and cellular differentiation.Methods SAT fatty acids and the expression of 117 genes were measured in 174 men and women from the DiOGenes Study using gas chromatography and qRT-PCR, respectively. Associations between fatty acids, gene expression, and SNPs in FADS1/FADS2 were investigated by linear regression and multivariate analysis.Results Four SNPs (rs174537, rs174546, rs174556, rs174601) in FADSConclusion This study showed that FADS1 genotype is distinguished by SAT fatty acid profiles, but not inflammatory gene expression.",

keywords = "DESATURASE ACTIVITY, ARACHIDONIC-ACID, DELTA-6, CLUSTER, HEALTH",

author = "Klingel, {Shannon L.} and Armand Valsesia and Arne Astrup and Marie Kunesova and Saris, {Wim H. M.} and Dominique Langin and Nathalie Viguerie and Mutch, {David M.}",

note = "Funding Information: Acknowledgements This work was supported by an Early Researcher Award from the Ministry of Research, Innovation and Science (Mutch) and the Commission of the European Communities (FP6-513946 DiOGenes). SLK is supported by a Queen Elizabeth II graduate student fellowship. DL is a member of Institut Universitaire de France. MK was supported by the grant MH CZ - DRO (Institute of Endocrinology - E{\'U}, 00023761). Publisher Copyright: {\textcopyright} 2018, Springer Nature Limited.",

year = "2019",

month = aug,

doi = "10.1038/s41366-018-0169-z",

language = "English",

volume = "43",

pages = "1539--1548",

journal = "International Journal of Obesity",

issn = "0307-0565",

publisher = "Nature Publishing Group",

number = "8",

}

TY - JOUR

T1 - FADS1 genotype is distinguished by human subcutaneous adipose tissue fatty acids, but not inflammatory gene expression

AU - Klingel, Shannon L.

AU - Valsesia, Armand

AU - Astrup, Arne

AU - Kunesova, Marie

AU - Saris, Wim H. M.

AU - Langin, Dominique

AU - Viguerie, Nathalie

AU - Mutch, David M.

N1 - Funding Information: Acknowledgements This work was supported by an Early Researcher Award from the Ministry of Research, Innovation and Science (Mutch) and the Commission of the European Communities (FP6-513946 DiOGenes). SLK is supported by a Queen Elizabeth II graduate student fellowship. DL is a member of Institut Universitaire de France. MK was supported by the grant MH CZ - DRO (Institute of Endocrinology - EÚ, 00023761). Publisher Copyright: © 2018, Springer Nature Limited.

PY - 2019/8

Y1 - 2019/8

N2 - Background Single nucleotide polymorphisms (SNPs) in FADS1/FADS2 genes are associated with changes in serum and tissue polyunsaturated fatty acid (PUFA) content. PUFA regulate inflammatory signaling pathways in adipose tissue; however, the effect of SNPs in FADS1/FADS2 on adipose tissue inflammation is equivocal. The present study examined if SNPs in FADS1/FADS2 modify human subcutaneous adipose tissue (SAT) fatty acid profiles and the expression of genes associated with inflammation/immune function, lipid metabolism, and cellular differentiation.Methods SAT fatty acids and the expression of 117 genes were measured in 174 men and women from the DiOGenes Study using gas chromatography and qRT-PCR, respectively. Associations between fatty acids, gene expression, and SNPs in FADS1/FADS2 were investigated by linear regression and multivariate analysis.Results Four SNPs (rs174537, rs174546, rs174556, rs174601) in FADSConclusion This study showed that FADS1 genotype is distinguished by SAT fatty acid profiles, but not inflammatory gene expression.

AB - Background Single nucleotide polymorphisms (SNPs) in FADS1/FADS2 genes are associated with changes in serum and tissue polyunsaturated fatty acid (PUFA) content. PUFA regulate inflammatory signaling pathways in adipose tissue; however, the effect of SNPs in FADS1/FADS2 on adipose tissue inflammation is equivocal. The present study examined if SNPs in FADS1/FADS2 modify human subcutaneous adipose tissue (SAT) fatty acid profiles and the expression of genes associated with inflammation/immune function, lipid metabolism, and cellular differentiation.Methods SAT fatty acids and the expression of 117 genes were measured in 174 men and women from the DiOGenes Study using gas chromatography and qRT-PCR, respectively. Associations between fatty acids, gene expression, and SNPs in FADS1/FADS2 were investigated by linear regression and multivariate analysis.Results Four SNPs (rs174537, rs174546, rs174556, rs174601) in FADSConclusion This study showed that FADS1 genotype is distinguished by SAT fatty acid profiles, but not inflammatory gene expression.

KW - DESATURASE ACTIVITY

KW - ARACHIDONIC-ACID

KW - DELTA-6

KW - CLUSTER

KW - HEALTH

U2 - 10.1038/s41366-018-0169-z

DO - 10.1038/s41366-018-0169-z

M3 - Article

C2 - 30082751

SN - 0307-0565

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SP - 1539

EP - 1548

JO - International Journal of Obesity

JF - International Journal of Obesity

IS - 8

ER -