Elevated citrate levels in non-alcoholic fatty liver disease: The potential of citrate to promote radical production

B. van de Wier; J.M. Balk; G.R.M.M. Haenen; D. Giamouridis; J.A. Bakker; B.C. Bast; G.J.M. den Hartog; G.H. Koek; A. Bast

doi:10.1016/j.febslet.2013.06.019

Elevated citrate levels in non-alcoholic fatty liver disease: The potential of citrate to promote radical production

B. van de Wier^*, J.M. Balk, G.R.M.M. Haenen, D. Giamouridis, J.A. Bakker, B.C. Bast, G.J.M. den Hartog, G.H. Koek, A. Bast

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Plasma citrate levels were found to be elevated in non-alcoholic fatty liver disease (NAFLD) patients. Cellular experiments indicated that increased citrate levels might originate from an excess of fatty acids. The impact of elevated citrate levels on oxidative stress was examined. It was found that citrate stimulated hydrogen peroxide induced intracellular oxidative stress in HepG2 cells. This was related to the promotion of iron mediated hydroxyl radical formation from hydrogen peroxide by citrate. The stimulating effect of citrate on the reactivity of iron promotes oxidative stress, a crucial process in the progression of NAFLD.

Original language	English
Pages (from-to)	2461-2466
Number of pages	6
Journal	Febs Letters
Volume	587
Issue number	15
DOIs	https://doi.org/10.1016/j.febslet.2013.06.019
Publication status	Published - 2 Aug 2013

Keywords

Citrate
Non-alcoholic fatty liver disease
Oxidative stress
Fenton reaction
Hydroxyl radical
Iron
MICROSOMAL LIPID-PEROXIDATION
HYDROXYL RADICALS
OXIDATIVE STRESS
IRON CHELATORS
CITRIC-ACID
STEATOHEPATITIS
COMPLEXES
FERRITIN
DAMAGE

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10.1016/j.febslet.2013.06.019

Cite this

@article{cab0a81ec99141409319df19e1ba1c07,

title = "Elevated citrate levels in non-alcoholic fatty liver disease: The potential of citrate to promote radical production",

abstract = "Plasma citrate levels were found to be elevated in non-alcoholic fatty liver disease (NAFLD) patients. Cellular experiments indicated that increased citrate levels might originate from an excess of fatty acids. The impact of elevated citrate levels on oxidative stress was examined. It was found that citrate stimulated hydrogen peroxide induced intracellular oxidative stress in HepG2 cells. This was related to the promotion of iron mediated hydroxyl radical formation from hydrogen peroxide by citrate. The stimulating effect of citrate on the reactivity of iron promotes oxidative stress, a crucial process in the progression of NAFLD.",

keywords = "Citrate, Non-alcoholic fatty liver disease, Oxidative stress, Fenton reaction, Hydroxyl radical, Iron, MICROSOMAL LIPID-PEROXIDATION, HYDROXYL RADICALS, OXIDATIVE STRESS, IRON CHELATORS, CITRIC-ACID, STEATOHEPATITIS, COMPLEXES, FERRITIN, DAMAGE",

author = "{van de Wier}, B. and J.M. Balk and G.R.M.M. Haenen and D. Giamouridis and J.A. Bakker and B.C. Bast and {den Hartog}, G.J.M. and G.H. Koek and A. Bast",

year = "2013",

month = aug,

day = "2",

doi = "10.1016/j.febslet.2013.06.019",

language = "English",

volume = "587",

pages = "2461--2466",

journal = "Febs Letters",

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T1 - Elevated citrate levels in non-alcoholic fatty liver disease: The potential of citrate to promote radical production

AU - van de Wier, B.

AU - Balk, J.M.

AU - Haenen, G.R.M.M.

AU - Giamouridis, D.

AU - Bakker, J.A.

AU - Bast, B.C.

AU - den Hartog, G.J.M.

AU - Koek, G.H.

AU - Bast, A.

PY - 2013/8/2

Y1 - 2013/8/2

N2 - Plasma citrate levels were found to be elevated in non-alcoholic fatty liver disease (NAFLD) patients. Cellular experiments indicated that increased citrate levels might originate from an excess of fatty acids. The impact of elevated citrate levels on oxidative stress was examined. It was found that citrate stimulated hydrogen peroxide induced intracellular oxidative stress in HepG2 cells. This was related to the promotion of iron mediated hydroxyl radical formation from hydrogen peroxide by citrate. The stimulating effect of citrate on the reactivity of iron promotes oxidative stress, a crucial process in the progression of NAFLD.

AB - Plasma citrate levels were found to be elevated in non-alcoholic fatty liver disease (NAFLD) patients. Cellular experiments indicated that increased citrate levels might originate from an excess of fatty acids. The impact of elevated citrate levels on oxidative stress was examined. It was found that citrate stimulated hydrogen peroxide induced intracellular oxidative stress in HepG2 cells. This was related to the promotion of iron mediated hydroxyl radical formation from hydrogen peroxide by citrate. The stimulating effect of citrate on the reactivity of iron promotes oxidative stress, a crucial process in the progression of NAFLD.

KW - Citrate

KW - Non-alcoholic fatty liver disease

KW - Oxidative stress

KW - Fenton reaction

KW - Hydroxyl radical

KW - Iron

KW - MICROSOMAL LIPID-PEROXIDATION

KW - HYDROXYL RADICALS

KW - OXIDATIVE STRESS

KW - IRON CHELATORS

KW - CITRIC-ACID

KW - STEATOHEPATITIS

KW - COMPLEXES

KW - FERRITIN

KW - DAMAGE

U2 - 10.1016/j.febslet.2013.06.019

DO - 10.1016/j.febslet.2013.06.019

M3 - Article

C2 - 23792160

SN - 0014-5793

VL - 587

SP - 2461

EP - 2466

JO - Febs Letters

JF - Febs Letters

IS - 15

ER -