TY - JOUR
T1 - Effects of Glucagon-Like Peptide-2-Expressing Saccharomyces cerevisiae Not Different from Empty Vector
AU - Zhong, Xi
AU - Liang, Guopeng
AU - Cao, Lili
AU - Qiao, Qi
AU - Hu, Zhi
AU - Fu, Min
AU - Bo, Hong
AU - Wu, Qin
AU - Liang, Guanlin
AU - Zhang, Zhongwei
AU - Zhou, Lin
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (31301116), and the Maj or Proj ect of the Education Department in Sichuan (11ZA297).
Funding Information:
This work was supported by the National Natural Science Foundation of China (31301116), and the Major Project of the Education Department in Sichuan (11ZA297).
Publisher Copyright:
Copyright© 2019 by The Korean Society for Microbiology and Biotechnology.
PY - 2019/10
Y1 - 2019/10
N2 - Saccharomyces cerevisiae (S. cerevisiae) and glucagon-like peptide-2 (GLP-2) have been employed to improve the intestinal development of weaned animals. The goal of this study was to determine whether either exogenous S. cerevisiae or GLP-2 elicits major effects on fecal microbiotas and cytokine responses in weaned piglets. Ninety-six piglets weaned at 26 days were assigned to one of four groups: 1) Basal diet (Control), 2) empty vector-harboring S. cerevisiae (EV-SC), 3) GLP-2-expressing S. cerevisiae (GLP2-SC), and 4) recombinant human GLP-2 (rh-GLP2). At the start of the post-weaning period (day 0), and at day 28, fecal samples were collected to assess the bacterial communities via sequencing the V1-V2 region of the 16S-rRNA gene, and piglets' blood was also sampled to measure cytokine responses (i.e., IL-1 beta, TNF-alpha, and IFN-gamma). This study revealed that, on the one hand, although S. cerevisiae supplementation did not significantly alter the growth of weaned piglets, it induced increases in the relative abundances of two core genera (Ruminococcaceae_norank and Erysipelotrichaceae_norank) and decreases in the relative abundances of two other core genera (Lachnospiraceae_norank and Clostridiale_norank) and cytokine levels (IL-1 beta and TNF-alpha) (p <0.05, Control vs EV-SC; p <0.05, rh-GLP2 vs GLP2-SC). On the other hand, GLP-2 supplementation had no significant influence on fecal bacterial communities and cytokine levels, but it produced better body weight and average daily gain (p <0.05, Control vs EV-SC; p <0.05, rh-GLP2 vs GLP2-SC). Therefore, altered fecal microbiotas and cytokine response effects in weaned piglets were due to S. cerevisiae rather than GLP-2.
AB - Saccharomyces cerevisiae (S. cerevisiae) and glucagon-like peptide-2 (GLP-2) have been employed to improve the intestinal development of weaned animals. The goal of this study was to determine whether either exogenous S. cerevisiae or GLP-2 elicits major effects on fecal microbiotas and cytokine responses in weaned piglets. Ninety-six piglets weaned at 26 days were assigned to one of four groups: 1) Basal diet (Control), 2) empty vector-harboring S. cerevisiae (EV-SC), 3) GLP-2-expressing S. cerevisiae (GLP2-SC), and 4) recombinant human GLP-2 (rh-GLP2). At the start of the post-weaning period (day 0), and at day 28, fecal samples were collected to assess the bacterial communities via sequencing the V1-V2 region of the 16S-rRNA gene, and piglets' blood was also sampled to measure cytokine responses (i.e., IL-1 beta, TNF-alpha, and IFN-gamma). This study revealed that, on the one hand, although S. cerevisiae supplementation did not significantly alter the growth of weaned piglets, it induced increases in the relative abundances of two core genera (Ruminococcaceae_norank and Erysipelotrichaceae_norank) and decreases in the relative abundances of two other core genera (Lachnospiraceae_norank and Clostridiale_norank) and cytokine levels (IL-1 beta and TNF-alpha) (p <0.05, Control vs EV-SC; p <0.05, rh-GLP2 vs GLP2-SC). On the other hand, GLP-2 supplementation had no significant influence on fecal bacterial communities and cytokine levels, but it produced better body weight and average daily gain (p <0.05, Control vs EV-SC; p <0.05, rh-GLP2 vs GLP2-SC). Therefore, altered fecal microbiotas and cytokine response effects in weaned piglets were due to S. cerevisiae rather than GLP-2.
KW - Sus scrofa
KW - weaned piglets
KW - Saccharomyces cerevisiae
KW - glucagon-like peptide-2
KW - fecal microbiotas
KW - GROWTH-PERFORMANCE
KW - INTESTINAL DEVELOPMENT
KW - BIOLOGICAL-ACTIVITIES
KW - INTRACELLULAR EGF
KW - EXTRACELLULAR EGF
KW - TAGGED EGF
KW - PEPTIDE 2
KW - YEAST
KW - IMMUNITY
KW - CELL
U2 - 10.4014/jmb.1907.07006
DO - 10.4014/jmb.1907.07006
M3 - Article
C2 - 31474096
SN - 1017-7825
VL - 29
SP - 1644
EP - 1655
JO - Journal of Microbiology and Biotechnology
JF - Journal of Microbiology and Biotechnology
IS - 10
ER -