Discovering new pathways in thrombus formation

Platelets play crucial role in hemostasis and thrombosis and their improper function can lead to either bleeding tendencies or occlusive thrombus formation. Applying an in vitro flow chamber technique, the role of platelets in regulation of thrombus formation was investigated in interaction with the blood plasma, leukocytes and vessel wall components in order to better understand the underlying mechanisms. This effort led to the identification of altered platelet functions in immune deficient patients and genetically modified mice. By the development and application of standardized multiparameter analysis of thrombus formation, the antithrombotic profile of genetically modified mice was determined in relation to the GPVI activity. Furthermore, it also identified new regulatory mechanism in platelet-dependent leukocyte activation and unraveled new type of structure implicated in platelet-platelet interaction.