TY - JOUR
T1 - Detection of Peptide-Based Nanoparticles in Blood Plasma by ELISA
AU - Bode, Gerard H.
AU - Pickl, Karin E.
AU - Sanchez-Purra, Maria
AU - Albaiges, Berta
AU - Borros, Salvador
AU - Poetgens, Andy J. G.
AU - Schmitz, Christoph
AU - Sinner, Frank M.
AU - Losen, Mario
AU - Steinbusch, Harry W. M.
AU - Frank, Hans-Georg
AU - Martinez-Martinez, Pilar
PY - 2015/5/21
Y1 - 2015/5/21
N2 - Aims The aim of the current study was to develop a method to detect peptide-linked nanoparticles in blood plasma. Materials & Methods A convenient enzyme linked immunosorbent assay (ELISA) was developed for the detection of peptides functionalized with biotin and fluorescein groups. As a proof of principle, polymerized pentafluorophenyl methacrylate nanoparticles linked to biotin-carboxyfluorescein labeled peptides were intravenously injected in Wistar rats. Serial blood plasma samples were analyzed by ELISA and by liquid chromatography mass spectrometry (LC/MS) technology. Results The ELISA based method for the detection of FITC labeled peptides had a detection limit of 1 ng/mL. We were able to accurately measure peptides bound to pentafluorophenyl methacrylate nanoparticles in blood plasma of rats, and similar results were obtained by LC/MS. Conclusions We detected FITC-labeled peptides on pentafluorophenyl methacrylate nanoparticles after injection in vivo. This method can be extended to detect nanoparticles with different chemical compositions.
AB - Aims The aim of the current study was to develop a method to detect peptide-linked nanoparticles in blood plasma. Materials & Methods A convenient enzyme linked immunosorbent assay (ELISA) was developed for the detection of peptides functionalized with biotin and fluorescein groups. As a proof of principle, polymerized pentafluorophenyl methacrylate nanoparticles linked to biotin-carboxyfluorescein labeled peptides were intravenously injected in Wistar rats. Serial blood plasma samples were analyzed by ELISA and by liquid chromatography mass spectrometry (LC/MS) technology. Results The ELISA based method for the detection of FITC labeled peptides had a detection limit of 1 ng/mL. We were able to accurately measure peptides bound to pentafluorophenyl methacrylate nanoparticles in blood plasma of rats, and similar results were obtained by LC/MS. Conclusions We detected FITC-labeled peptides on pentafluorophenyl methacrylate nanoparticles after injection in vivo. This method can be extended to detect nanoparticles with different chemical compositions.
U2 - 10.1371/journal.pone.0126136
DO - 10.1371/journal.pone.0126136
M3 - Article
C2 - 25996618
SN - 1932-6203
VL - 10
JO - PLOS ONE
JF - PLOS ONE
IS - 5
M1 - e0126136
ER -