Detection of Intraplaque Hemorrhage in Mouse Atherosclerotic Lesions

Judith C Sluimer; Marion J Gijbels; Sylvia Heeneman

doi:10.1007/978-1-4939-2929-0_24

Detection of Intraplaque Hemorrhage in Mouse Atherosclerotic Lesions

Judith C Sluimer, Marion J Gijbels, Sylvia Heeneman

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Intraplaque hemorrhage is defined as the presence of fresh or lysed erythrocytes, iron deposits in macrophages, and/or a fibrin clot in an atherosclerotic plaque. These features can be detected by hematoxylin and eosin, Martius scarlet Blue, and Perl's iron histological stainings. It is noteworthy that intraplaque hemorrhage is only present in murine atherosclerotic plaques after additional interventions or additional genetic traits affecting matrix degradation or thrombosis. In this chapter, we describe methods to detect intraplaque hemorrhage in mouse atherosclerotic lesions.

Original language	English
Pages (from-to)	339-48
Number of pages	10
Journal	Methods in Molecular Biology
Volume	1339
DOIs	https://doi.org/10.1007/978-1-4939-2929-0_24
Publication status	Published - 2015

Keywords

Animals
Aorta
Aortic Diseases
Apolipoproteins E
Atherosclerosis
Azo Compounds
Coloring Agents
Disease Models, Animal
Eosine Yellowish-(YS)
Ferrocyanides
Genetic Predisposition to Disease
Hematoxylin
Hemorrhage
Mice
Mice, Knockout
Phenotype
Plaque, Atherosclerotic
Staining and Labeling

Access to Document

10.1007/978-1-4939-2929-0_24

Cite this

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title = "Detection of Intraplaque Hemorrhage in Mouse Atherosclerotic Lesions",

abstract = "Intraplaque hemorrhage is defined as the presence of fresh or lysed erythrocytes, iron deposits in macrophages, and/or a fibrin clot in an atherosclerotic plaque. These features can be detected by hematoxylin and eosin, Martius scarlet Blue, and Perl's iron histological stainings. It is noteworthy that intraplaque hemorrhage is only present in murine atherosclerotic plaques after additional interventions or additional genetic traits affecting matrix degradation or thrombosis. In this chapter, we describe methods to detect intraplaque hemorrhage in mouse atherosclerotic lesions.",

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journal = "Methods in Molecular Biology",

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AU - Sluimer, Judith C

AU - Gijbels, Marion J

AU - Heeneman, Sylvia

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Y1 - 2015

N2 - Intraplaque hemorrhage is defined as the presence of fresh or lysed erythrocytes, iron deposits in macrophages, and/or a fibrin clot in an atherosclerotic plaque. These features can be detected by hematoxylin and eosin, Martius scarlet Blue, and Perl's iron histological stainings. It is noteworthy that intraplaque hemorrhage is only present in murine atherosclerotic plaques after additional interventions or additional genetic traits affecting matrix degradation or thrombosis. In this chapter, we describe methods to detect intraplaque hemorrhage in mouse atherosclerotic lesions.

AB - Intraplaque hemorrhage is defined as the presence of fresh or lysed erythrocytes, iron deposits in macrophages, and/or a fibrin clot in an atherosclerotic plaque. These features can be detected by hematoxylin and eosin, Martius scarlet Blue, and Perl's iron histological stainings. It is noteworthy that intraplaque hemorrhage is only present in murine atherosclerotic plaques after additional interventions or additional genetic traits affecting matrix degradation or thrombosis. In this chapter, we describe methods to detect intraplaque hemorrhage in mouse atherosclerotic lesions.

KW - Animals

KW - Aorta

KW - Aortic Diseases

KW - Apolipoproteins E

KW - Atherosclerosis

KW - Azo Compounds

KW - Coloring Agents

KW - Disease Models, Animal

KW - Eosine Yellowish-(YS)

KW - Ferrocyanides

KW - Genetic Predisposition to Disease

KW - Hematoxylin

KW - Hemorrhage

KW - Mice

KW - Mice, Knockout

KW - Phenotype

KW - Plaque, Atherosclerotic

KW - Staining and Labeling

U2 - 10.1007/978-1-4939-2929-0_24

DO - 10.1007/978-1-4939-2929-0_24

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C2 - 26445801

SN - 1064-3745

VL - 1339

SP - 339

EP - 348

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

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