Research output

Delivery of DNA into the central nervous system via electroporation

Research output: Chapter in Book/Report/Conference proceedingChapterAcademic

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Abstract

Electroporation of non-viral plasmid DNA is a valuable tool to alter gene expression in the adult central nervous system. It offers a number of advantages over viral gene delivery as non-viral plasmids can integrate larger inserts and reduce the risk of inducing unintended immunological responses. Generally, electroporation of the adult brain is accomplished in rodents by applying high-amplitude voltage-controlled pulses through the entire brain with plate electrodes surrounding the animal's head. Here, we describe an alternative electroporation protocol making use of current-controlled low-amplitude pulses that are delivered locally by means of needlelike electrodes in the brain of adult mice. This allows altering gene expression in very-well-defined areas of the brain while inducing minimal tissue damage.

    Research areas

  • Analgesia, Animals, Central Nervous System, DNA, Electroporation, Injections, Mice, Mice, Inbred C57BL, Stereotaxic Techniques, Transfection
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Details

Original languageEnglish
Title of host publicationMethods in molecular biology
Pages157-63
Number of pages7
Volume1121
DOIs
Publication statusPublished - 2014