Ciona intestinalis NADH dehydrogenase NDX confers stress-resistance and extended lifespan on Drosophila

Dmytro V. Gospodaryov; Oleh V. Lushchak; Bohdana M. Rovenko; Natalia V. Perkhulyn; Mike Gerards; Tea Tuomela; Howard T. Jacobs

doi:10.1016/j.bbabio.2014.08.001

Ciona intestinalis NADH dehydrogenase NDX confers stress-resistance and extended lifespan on Drosophila

Dmytro V. Gospodaryov, Oleh V. Lushchak, Bohdana M. Rovenko, Natalia V. Perkhulyn, Mike Gerards, Tea Tuomela, Howard T. Jacobs^*

^*Corresponding author for this work

GROW - Reproductive and Perinatal Medicine

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

An assembled cDNA coding for the putative single-subunit NADH dehydrogenase (NDX) of Ciona intestinalis was introduced into Drosophila melanogaster. The encoded protein was found to localize to mitochondria and to confer rotenone-insensitive substrate oxidation in organello. Transgenic flies exhibited increased resistance to menadione, starvation and temperature stress, and manifested a sex and diet-dependent increase in mean lifespan of 20-50%. However, NDX was able only weakly to complement the phenotypes produced by the knockdown of complex I subunits. Published by Elsevier B.V.

Original language	English
Pages (from-to)	1861-1869
Journal	Biochimica et Biophysica Acta-bioenergetics
Volume	1837
Issue number	11
DOIs	https://doi.org/10.1016/j.bbabio.2014.08.001
Publication status	Published - Nov 2014

Keywords

Alternative respiratory chain enzymes
Tunicate
Gene therapy
Oxidative stress
Ageing

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10.1016/j.bbabio.2014.08.001Licence: CC BY-NC-ND

Cite this

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title = "Ciona intestinalis NADH dehydrogenase NDX confers stress-resistance and extended lifespan on Drosophila",

abstract = "An assembled cDNA coding for the putative single-subunit NADH dehydrogenase (NDX) of Ciona intestinalis was introduced into Drosophila melanogaster. The encoded protein was found to localize to mitochondria and to confer rotenone-insensitive substrate oxidation in organello. Transgenic flies exhibited increased resistance to menadione, starvation and temperature stress, and manifested a sex and diet-dependent increase in mean lifespan of 20-50%. However, NDX was able only weakly to complement the phenotypes produced by the knockdown of complex I subunits. Published by Elsevier B.V.",

keywords = "Alternative respiratory chain enzymes, Tunicate, Gene therapy, Oxidative stress, Ageing",

author = "Gospodaryov, {Dmytro V.} and Lushchak, {Oleh V.} and Rovenko, {Bohdana M.} and Perkhulyn, {Natalia V.} and Mike Gerards and Tea Tuomela and Jacobs, {Howard T.}",

year = "2014",

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doi = "10.1016/j.bbabio.2014.08.001",

language = "English",

volume = "1837",

pages = "1861--1869",

journal = "Biochimica et Biophysica Acta-bioenergetics",

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T1 - Ciona intestinalis NADH dehydrogenase NDX confers stress-resistance and extended lifespan on Drosophila

AU - Gospodaryov, Dmytro V.

AU - Lushchak, Oleh V.

AU - Rovenko, Bohdana M.

AU - Perkhulyn, Natalia V.

AU - Gerards, Mike

AU - Tuomela, Tea

AU - Jacobs, Howard T.

PY - 2014/11

Y1 - 2014/11

N2 - An assembled cDNA coding for the putative single-subunit NADH dehydrogenase (NDX) of Ciona intestinalis was introduced into Drosophila melanogaster. The encoded protein was found to localize to mitochondria and to confer rotenone-insensitive substrate oxidation in organello. Transgenic flies exhibited increased resistance to menadione, starvation and temperature stress, and manifested a sex and diet-dependent increase in mean lifespan of 20-50%. However, NDX was able only weakly to complement the phenotypes produced by the knockdown of complex I subunits. Published by Elsevier B.V.

AB - An assembled cDNA coding for the putative single-subunit NADH dehydrogenase (NDX) of Ciona intestinalis was introduced into Drosophila melanogaster. The encoded protein was found to localize to mitochondria and to confer rotenone-insensitive substrate oxidation in organello. Transgenic flies exhibited increased resistance to menadione, starvation and temperature stress, and manifested a sex and diet-dependent increase in mean lifespan of 20-50%. However, NDX was able only weakly to complement the phenotypes produced by the knockdown of complex I subunits. Published by Elsevier B.V.

KW - Alternative respiratory chain enzymes

KW - Tunicate

KW - Gene therapy

KW - Oxidative stress

KW - Ageing

U2 - 10.1016/j.bbabio.2014.08.001

DO - 10.1016/j.bbabio.2014.08.001

M3 - Article

C2 - 25124484

SN - 0005-2728

VL - 1837

SP - 1861

EP - 1869

JO - Biochimica et Biophysica Acta-bioenergetics

JF - Biochimica et Biophysica Acta-bioenergetics

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