Characterization of imipenem resistance mechanisms in Pseudomonas aeruginosa isolates from Turkey

M. Mac Aogain; C. Kulah; M. Rijnsburger; G. Celebi; P.H. Savelkoul; F. O'Gara; M.J. Mooij

doi:10.1111/j.1469-0691.2012.03899.x

Characterization of imipenem resistance mechanisms in Pseudomonas aeruginosa isolates from Turkey

M. Mac Aogain, C. Kulah, M. Rijnsburger, G. Celebi, P.H. Savelkoul, F. O'Gara^*, M.J. Mooij

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The emergence of carbapenem resistance in Pseudomonas aeruginosa threatens the efficacy of this important anti-pseudomonal antibiotic class. Between 2003 and 2006, an increase in the number of carbapenem-resistant P. aeruginosa isolates at the Zonguldak Karaelmas University Hospital was observed (Zonguldak, Turkey). To assess the imipenem resistance mechanisms emerging in these P. aeruginosa isolates, they were characterized by amplified fragment length polymorphism typing, which revealed diversity among imipenem-resistant isolates as well as two clonally related outbreak groups. The molecular mechanism of carbapenem resistance was characterized in a representative isolate from each clonal group. Mutational disruption of oprD was the most frequently encountered resistance mechanism (23/27 isolates).

Original language	English
Pages (from-to)	E262-E265
Number of pages	4
Journal	Clinical Microbiology and Infection
Volume	18
Issue number	7
DOIs	https://doi.org/10.1111/j.1469-0691.2012.03899.x
Publication status	Published - Jul 2012

Keywords

Imipenem
oprD
Pseudomonas aeruginosa
Turkey
NOSOCOMIAL INFECTIONS
REGULATOR
STRAINS
MEXT

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10.1111/j.1469-0691.2012.03899.xLicence: Free access - publisher

Cite this

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title = "Characterization of imipenem resistance mechanisms in Pseudomonas aeruginosa isolates from Turkey",

abstract = "The emergence of carbapenem resistance in Pseudomonas aeruginosa threatens the efficacy of this important anti-pseudomonal antibiotic class. Between 2003 and 2006, an increase in the number of carbapenem-resistant P. aeruginosa isolates at the Zonguldak Karaelmas University Hospital was observed (Zonguldak, Turkey). To assess the imipenem resistance mechanisms emerging in these P. aeruginosa isolates, they were characterized by amplified fragment length polymorphism typing, which revealed diversity among imipenem-resistant isolates as well as two clonally related outbreak groups. The molecular mechanism of carbapenem resistance was characterized in a representative isolate from each clonal group. Mutational disruption of oprD was the most frequently encountered resistance mechanism (23/27 isolates).",

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author = "{Mac Aogain}, M. and C. Kulah and M. Rijnsburger and G. Celebi and P.H. Savelkoul and F. O'Gara and M.J. Mooij",

year = "2012",

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T1 - Characterization of imipenem resistance mechanisms in Pseudomonas aeruginosa isolates from Turkey

AU - Mac Aogain, M.

AU - Kulah, C.

AU - Rijnsburger, M.

AU - Celebi, G.

AU - Savelkoul, P.H.

AU - O'Gara, F.

AU - Mooij, M.J.

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AB - The emergence of carbapenem resistance in Pseudomonas aeruginosa threatens the efficacy of this important anti-pseudomonal antibiotic class. Between 2003 and 2006, an increase in the number of carbapenem-resistant P. aeruginosa isolates at the Zonguldak Karaelmas University Hospital was observed (Zonguldak, Turkey). To assess the imipenem resistance mechanisms emerging in these P. aeruginosa isolates, they were characterized by amplified fragment length polymorphism typing, which revealed diversity among imipenem-resistant isolates as well as two clonally related outbreak groups. The molecular mechanism of carbapenem resistance was characterized in a representative isolate from each clonal group. Mutational disruption of oprD was the most frequently encountered resistance mechanism (23/27 isolates).

KW - Imipenem

KW - oprD

KW - Pseudomonas aeruginosa

KW - Turkey

KW - NOSOCOMIAL INFECTIONS

KW - REGULATOR

KW - STRAINS

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