Abstract
A straightforward protocol for the covalent functionalization of boron-doped diamond electrodes with either ferrocene or single-stranded deoxyribonucleic acid (DNA) is reported. The functionalization method is based on a combination of diazonium salt electrografting and click chemistry. An azide-terminated organic layer is first electrografted onto the diamond surface by electrochemical reduction of 4-azidophenyldiazonium chloride. The azidophenyl-modified surface then reacts rapidly and efficiently with molecules bearing a terminal alkyne moiety by means of Cu-1-catalyzed alkyne-azide cycloaddition. Covalent attachment of ferrocene moieties was analyzed by X-ray photoelectron spectroscopy and cyclic voltammetry, whereas impedance spectroscopy was applied for the characterization of the immobilized DNA.
Original language | English |
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Pages (from-to) | 1145-1154 |
Number of pages | 10 |
Journal | ChemElectroChem |
Volume | 1 |
Issue number | 7 |
DOIs | |
Publication status | Published - 15 Jul 2014 |
Keywords
- azides
- boron-doped diamond
- click chemistry
- diazonium electrografting
- DNA
- SELF-ASSEMBLED MONOLAYERS
- THIN-FILMS
- ULTRANANOCRYSTALLINE DIAMOND
- IMPEDANCE SPECTROSCOPY
- BIOSENSOR APPLICATIONS
- ELECTRICAL-PROPERTIES
- MOLECULAR MONOLAYERS
- COVALENT ATTACHMENT
- CONDUCTIVE DIAMOND
- CVD DIAMOND