Bacterial adherence to graft tissues in static and flow conditions

Tiago Rafael Veloso; Jorien Claes; Soetkin Van Kerckhoven; Bartosz Ditkowski; Luis G. Hurtado-Aguilar; Stefan Jockenhoevel; Petra Mela; Ramadan Jashari; Marc Gewillig; Marc F. Hoylaerts; Bart Meyns; Ruth Heying

doi:10.1016/j.jtcvs.2017.06.014

Bacterial adherence to graft tissues in static and flow conditions

Tiago Rafael Veloso, Jorien Claes, Soetkin Van Kerckhoven, Bartosz Ditkowski, Luis G. Hurtado-Aguilar, Stefan Jockenhoevel, Petra Mela, Ramadan Jashari, Marc Gewillig, Marc F. Hoylaerts, Bart Meyns, Ruth Heying^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Various conduits and stent-mounted valves are used as pulmonary valve graft tissues for right ventricular outflow tract reconstruction with good hemodynamic results. Valve replacement carries an increased risk of infective endocarditis (IE). Recent observations have increased awareness of the risk of IE after transcatheter implantation of a stent-mounted bovine jugular vein valve. This study focused on the susceptibility of graft tissue surfaces to bacterial adherence as a potential risk factor for subsequent IE.Adhesion of Staphylococcus aureus, Staphylococcus epidermidis, and Streptococcus sanguinis to bovine pericardium (BP) patch, bovine jugular vein (BJV), and cryopreserved homograft (CH) tissues was quantified under static and shear stress conditions. Microscopic analysis and histology were performed to evaluate bacterial adhesion to matrix components.In general, similar bacteria numbers were recovered from CH and BJV tissue surfaces for all strains, especially in flow conditions. Static bacterial adhesion to the CH wall was lower for S sanguinis adhesion (P?

Original language	English
Pages (from-to)	325-332
Journal	Journal of Thoracic and Cardiovascular Surgery
Volume	155
Issue number	1
DOIs	https://doi.org/10.1016/j.jtcvs.2017.06.014
Publication status	Published - Jan 2018

Keywords

congenital heart disease
congenital heart disease surgery
endocarditis
prosthetic heart valve
valved conduit

Access to Document

10.1016/j.jtcvs.2017.06.014Licence: Free access - publisher

Cite this

@article{f0a9d09b5e4c4edc959da6974be9e265,

title = "Bacterial adherence to graft tissues in static and flow conditions",

abstract = "Various conduits and stent-mounted valves are used as pulmonary valve graft tissues for right ventricular outflow tract reconstruction with good hemodynamic results. Valve replacement carries an increased risk of infective endocarditis (IE). Recent observations have increased awareness of the risk of IE after transcatheter implantation of a stent-mounted bovine jugular vein valve. This study focused on the susceptibility of graft tissue surfaces to bacterial adherence as a potential risk factor for subsequent IE.Adhesion of Staphylococcus aureus, Staphylococcus epidermidis, and Streptococcus sanguinis to bovine pericardium (BP) patch, bovine jugular vein (BJV), and cryopreserved homograft (CH) tissues was quantified under static and shear stress conditions. Microscopic analysis and histology were performed to evaluate bacterial adhesion to matrix components.In general, similar bacteria numbers were recovered from CH and BJV tissue surfaces for all strains, especially in flow conditions. Static bacterial adhesion to the CH wall was lower for S sanguinis adhesion (P?",

keywords = "congenital heart disease, congenital heart disease surgery, endocarditis, prosthetic heart valve, valved conduit",

author = "Veloso, {Tiago Rafael} and Jorien Claes and {Van Kerckhoven}, Soetkin and Bartosz Ditkowski and Hurtado-Aguilar, {Luis G.} and Stefan Jockenhoevel and Petra Mela and Ramadan Jashari and Marc Gewillig and Hoylaerts, {Marc F.} and Bart Meyns and Ruth Heying",

year = "2018",

month = jan,

doi = "10.1016/j.jtcvs.2017.06.014",

language = "English",

volume = "155",

pages = "325--332",

journal = "Journal of Thoracic and Cardiovascular Surgery",

issn = "0022-5223",

publisher = "MOSBY-ELSEVIER",

number = "1",

}

TY - JOUR

T1 - Bacterial adherence to graft tissues in static and flow conditions

AU - Veloso, Tiago Rafael

AU - Claes, Jorien

AU - Van Kerckhoven, Soetkin

AU - Ditkowski, Bartosz

AU - Hurtado-Aguilar, Luis G.

AU - Jockenhoevel, Stefan

AU - Mela, Petra

AU - Jashari, Ramadan

AU - Gewillig, Marc

AU - Hoylaerts, Marc F.

AU - Meyns, Bart

AU - Heying, Ruth

PY - 2018/1

Y1 - 2018/1

N2 - Various conduits and stent-mounted valves are used as pulmonary valve graft tissues for right ventricular outflow tract reconstruction with good hemodynamic results. Valve replacement carries an increased risk of infective endocarditis (IE). Recent observations have increased awareness of the risk of IE after transcatheter implantation of a stent-mounted bovine jugular vein valve. This study focused on the susceptibility of graft tissue surfaces to bacterial adherence as a potential risk factor for subsequent IE.Adhesion of Staphylococcus aureus, Staphylococcus epidermidis, and Streptococcus sanguinis to bovine pericardium (BP) patch, bovine jugular vein (BJV), and cryopreserved homograft (CH) tissues was quantified under static and shear stress conditions. Microscopic analysis and histology were performed to evaluate bacterial adhesion to matrix components.In general, similar bacteria numbers were recovered from CH and BJV tissue surfaces for all strains, especially in flow conditions. Static bacterial adhesion to the CH wall was lower for S sanguinis adhesion (P?

AB - Various conduits and stent-mounted valves are used as pulmonary valve graft tissues for right ventricular outflow tract reconstruction with good hemodynamic results. Valve replacement carries an increased risk of infective endocarditis (IE). Recent observations have increased awareness of the risk of IE after transcatheter implantation of a stent-mounted bovine jugular vein valve. This study focused on the susceptibility of graft tissue surfaces to bacterial adherence as a potential risk factor for subsequent IE.Adhesion of Staphylococcus aureus, Staphylococcus epidermidis, and Streptococcus sanguinis to bovine pericardium (BP) patch, bovine jugular vein (BJV), and cryopreserved homograft (CH) tissues was quantified under static and shear stress conditions. Microscopic analysis and histology were performed to evaluate bacterial adhesion to matrix components.In general, similar bacteria numbers were recovered from CH and BJV tissue surfaces for all strains, especially in flow conditions. Static bacterial adhesion to the CH wall was lower for S sanguinis adhesion (P?

KW - congenital heart disease

KW - congenital heart disease surgery

KW - endocarditis

KW - prosthetic heart valve

KW - valved conduit

U2 - 10.1016/j.jtcvs.2017.06.014

DO - 10.1016/j.jtcvs.2017.06.014

M3 - Article

SN - 0022-5223

VL - 155

SP - 325

EP - 332

JO - Journal of Thoracic and Cardiovascular Surgery

JF - Journal of Thoracic and Cardiovascular Surgery

IS - 1

ER -