TY - JOUR
T1 - Association of Kidney Function with Changes in the Endothelial Surface Layer
AU - Dane, Martijn J. C.
AU - Khairoun, Meriem
AU - Lee, Dae Hyun
AU - van den Berg, Bernard M.
AU - Eskens, Bart J. M.
AU - Boels, Margien G. S.
AU - van Teeffelen, Jurgen W. G. E.
AU - Rops, Angelique L. W. M. M.
AU - van der Vlag, Johan
AU - van Zonneveld, Anton Jan
AU - Reinders, Marlies E. J.
AU - Vink, Hans
AU - Rabelink, Ton J.
PY - 2014/4/7
Y1 - 2014/4/7
N2 - Background and objectivesESRD is accompanied by endothelial dysfunction. Because the endothelial glycocalyx (endothelial surface layer) governs interactions between flowing blood and the vessel wall, perturbation could influence disease progression. This study used a novel noninvasive sidestream-darkfield imaging method, which measures the accessibility of red blood cells to the endothelial surface layer in the microcirculation (perfused boundary region), to investigate whether renal function is associated with endothelial surface layer dimensions.Design, setting, participants, & measurementsPerfused boundary region was measured in control participants (n=10), patients with ESRD (n=23), participants with normal kidney function after successful living donor kidney transplantation (n=12), and patients who developed interstitial fibrosis/tubular atrophy after kidney transplantation (n=10). In addition, the endothelial activation marker angiopoietin-2 and shed endothelial surface layer components syndecan-1 and soluble thrombomodulin were measured using ELISA.ResultsCompared with healthy controls (1.820.16 mu m), ESRD patients had a larger perfused boundary region (+0.23; 95% confidence interval, 0.46 to
AB - Background and objectivesESRD is accompanied by endothelial dysfunction. Because the endothelial glycocalyx (endothelial surface layer) governs interactions between flowing blood and the vessel wall, perturbation could influence disease progression. This study used a novel noninvasive sidestream-darkfield imaging method, which measures the accessibility of red blood cells to the endothelial surface layer in the microcirculation (perfused boundary region), to investigate whether renal function is associated with endothelial surface layer dimensions.Design, setting, participants, & measurementsPerfused boundary region was measured in control participants (n=10), patients with ESRD (n=23), participants with normal kidney function after successful living donor kidney transplantation (n=12), and patients who developed interstitial fibrosis/tubular atrophy after kidney transplantation (n=10). In addition, the endothelial activation marker angiopoietin-2 and shed endothelial surface layer components syndecan-1 and soluble thrombomodulin were measured using ELISA.ResultsCompared with healthy controls (1.820.16 mu m), ESRD patients had a larger perfused boundary region (+0.23; 95% confidence interval, 0.46 to
U2 - 10.2215/CJN.08160813
DO - 10.2215/CJN.08160813
M3 - Article
C2 - 24458084
SN - 1555-9041
VL - 9
SP - 698
EP - 704
JO - Clinical journal of the American Society of Nephrology
JF - Clinical journal of the American Society of Nephrology
IS - 4
ER -