AnnexinA5-pHrodo: a new molecular probe for measuring efferocytosis

R. Stoehr; N. Deckers; L. Schurgers; N. Marx; C. P. Reutelingsperger

doi:10.1038/s41598-018-35995-z

AnnexinA5-pHrodo: a new molecular probe for measuring efferocytosis

R. Stoehr^*, N. Deckers, L. Schurgers, N. Marx, C. P. Reutelingsperger

^*Corresponding author for this work

Biochemie

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Efferocytosis, the clearing of dead or dying cells from living tissues, is a highly programmed, vital process to maintain the healthy functioning of every organism. Disorders of efferocytosis have been linked to several chronic diseases including atherosclerosis and auto-immune diseases. To date several different assays to determine phagocytosis, using microscopy or FACS analysis with labelled targets, have been developed. However, many of these are unable to differentiate between cells that have truly been phagocytosed and those still present on the surface of the macrophages hindering exact assessment of efferocytotic capacity. We herein describe AnxA5-pHrodo and its negative control M1234-pHrodo as new molecular probes to measure in vitro as well as ex-vivo efferocytotic capacity.

Original language	English
Article number	17731
Number of pages	9
Journal	Scientific Reports
Volume	8
DOIs	https://doi.org/10.1038/s41598-018-35995-z
Publication status	Published - 7 Dec 2018

Keywords

APOPTOTIC CELLS
ANNEXIN A5
PHOSPHATIDYLSERINE
PHAGOCYTOSIS
EXPOSURE
MACROPHAGES
ENGULFMENT
CLEARANCE
BINDING

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10.1038/s41598-018-35995-zLicence: CC BY

Cite this

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title = "AnnexinA5-pHrodo: a new molecular probe for measuring efferocytosis",

abstract = "Efferocytosis, the clearing of dead or dying cells from living tissues, is a highly programmed, vital process to maintain the healthy functioning of every organism. Disorders of efferocytosis have been linked to several chronic diseases including atherosclerosis and auto-immune diseases. To date several different assays to determine phagocytosis, using microscopy or FACS analysis with labelled targets, have been developed. However, many of these are unable to differentiate between cells that have truly been phagocytosed and those still present on the surface of the macrophages hindering exact assessment of efferocytotic capacity. We herein describe AnxA5-pHrodo and its negative control M1234-pHrodo as new molecular probes to measure in vitro as well as ex-vivo efferocytotic capacity.",

keywords = "APOPTOTIC CELLS, ANNEXIN A5, PHOSPHATIDYLSERINE, PHAGOCYTOSIS, EXPOSURE, MACROPHAGES, ENGULFMENT, CLEARANCE, BINDING",

author = "R. Stoehr and N. Deckers and L. Schurgers and N. Marx and Reutelingsperger, {C. P.}",

year = "2018",

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day = "7",

doi = "10.1038/s41598-018-35995-z",

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journal = "Scientific Reports",

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T1 - AnnexinA5-pHrodo

T2 - a new molecular probe for measuring efferocytosis

AU - Stoehr, R.

AU - Deckers, N.

AU - Schurgers, L.

AU - Marx, N.

AU - Reutelingsperger, C. P.

PY - 2018/12/7

Y1 - 2018/12/7

N2 - Efferocytosis, the clearing of dead or dying cells from living tissues, is a highly programmed, vital process to maintain the healthy functioning of every organism. Disorders of efferocytosis have been linked to several chronic diseases including atherosclerosis and auto-immune diseases. To date several different assays to determine phagocytosis, using microscopy or FACS analysis with labelled targets, have been developed. However, many of these are unable to differentiate between cells that have truly been phagocytosed and those still present on the surface of the macrophages hindering exact assessment of efferocytotic capacity. We herein describe AnxA5-pHrodo and its negative control M1234-pHrodo as new molecular probes to measure in vitro as well as ex-vivo efferocytotic capacity.

AB - Efferocytosis, the clearing of dead or dying cells from living tissues, is a highly programmed, vital process to maintain the healthy functioning of every organism. Disorders of efferocytosis have been linked to several chronic diseases including atherosclerosis and auto-immune diseases. To date several different assays to determine phagocytosis, using microscopy or FACS analysis with labelled targets, have been developed. However, many of these are unable to differentiate between cells that have truly been phagocytosed and those still present on the surface of the macrophages hindering exact assessment of efferocytotic capacity. We herein describe AnxA5-pHrodo and its negative control M1234-pHrodo as new molecular probes to measure in vitro as well as ex-vivo efferocytotic capacity.

KW - APOPTOTIC CELLS

KW - ANNEXIN A5

KW - PHOSPHATIDYLSERINE

KW - PHAGOCYTOSIS

KW - EXPOSURE

KW - MACROPHAGES

KW - ENGULFMENT

KW - CLEARANCE

KW - BINDING

U2 - 10.1038/s41598-018-35995-z

DO - 10.1038/s41598-018-35995-z

M3 - Article

C2 - 30532026

SN - 2045-2322

VL - 8

JO - Scientific Reports

JF - Scientific Reports

M1 - 17731

ER -