A Longitudinal Evaluation of Partial Lung Irradiation in Mice by Using a Dedicated Image-Guided Small Animal Irradiator

Patrick V. Granton; Ludwig Dubois; Wouter van Elmpt; Stefan J. van Hoof; Natasja G. Lieuwes; Dirk De Ruysscher; Frank Verhaegen

doi:10.1016/j.ijrobp.2014.07.004

A Longitudinal Evaluation of Partial Lung Irradiation in Mice by Using a Dedicated Image-Guided Small Animal Irradiator

Patrick V. Granton, Ludwig Dubois, Wouter van Elmpt, Stefan J. van Hoof, Natasja G. Lieuwes, Dirk De Ruysscher, Frank Verhaegen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Purpose: In lung cancer radiation therapy, the dose constraints are determined mostly by healthy lung toxicity. Preclinical microirradiators are a new tool to evaluate treatment strategies closer to clinical irradiation devices. In this study, we quantified local changes in lung density symptomatic of radiation-induced lung fibrosis (RILF) after partial lung irradiation in mice by using a precision image-guided small animal irradiator integrated with micro-computed tomography (CT) imaging. Methods and Materials: C57BL/6 adult male mice (n = 76) were divided into 6 groups: a control group (0 Gy) and groups irradiated with a single fraction of 4, 8, 12, 16, or 20 Gy using 5-mm circular parallel-opposed fields targeting the upper right lung. A Monte Carlo model of the small animal irradiator was used for dose calculations. Following irradiation, all mice were imaged at regular intervals over 39 weeks (10 time points total). Nonrigid deformation was used to register the initial micro-CT scan to all subsequent scans. Results: Significant differences could be observed between the 3 highest (>10 Gy) and 3 lowest irradiation (

Original language	English
Pages (from-to)	696-704
Journal	International Journal of Radiation Oncology Biology Physics
Volume	90
Issue number	3
DOIs	https://doi.org/10.1016/j.ijrobp.2014.07.004
Publication status	Published - 1 Nov 2014

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10.1016/j.ijrobp.2014.07.004

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@article{342be671f2fe4363b553804171b84366,

title = "A Longitudinal Evaluation of Partial Lung Irradiation in Mice by Using a Dedicated Image-Guided Small Animal Irradiator",

abstract = "Purpose: In lung cancer radiation therapy, the dose constraints are determined mostly by healthy lung toxicity. Preclinical microirradiators are a new tool to evaluate treatment strategies closer to clinical irradiation devices. In this study, we quantified local changes in lung density symptomatic of radiation-induced lung fibrosis (RILF) after partial lung irradiation in mice by using a precision image-guided small animal irradiator integrated with micro-computed tomography (CT) imaging. Methods and Materials: C57BL/6 adult male mice (n = 76) were divided into 6 groups: a control group (0 Gy) and groups irradiated with a single fraction of 4, 8, 12, 16, or 20 Gy using 5-mm circular parallel-opposed fields targeting the upper right lung. A Monte Carlo model of the small animal irradiator was used for dose calculations. Following irradiation, all mice were imaged at regular intervals over 39 weeks (10 time points total). Nonrigid deformation was used to register the initial micro-CT scan to all subsequent scans. Results: Significant differences could be observed between the 3 highest (>10 Gy) and 3 lowest irradiation (",

author = "Granton, {Patrick V.} and Ludwig Dubois and {van Elmpt}, Wouter and {van Hoof}, {Stefan J.} and Lieuwes, {Natasja G.} and {De Ruysscher}, Dirk and Frank Verhaegen",

year = "2014",

month = nov,

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doi = "10.1016/j.ijrobp.2014.07.004",

language = "English",

volume = "90",

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journal = "International Journal of Radiation Oncology Biology Physics",

issn = "0360-3016",

publisher = "Elsevier Science",

number = "3",

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A Longitudinal Evaluation of Partial Lung Irradiation in Mice by Using a Dedicated Image-Guided Small Animal Irradiator. / Granton, Patrick V.; Dubois, Ludwig ; van Elmpt, Wouter et al.
In: International Journal of Radiation Oncology Biology Physics, Vol. 90, No. 3, 01.11.2014, p. 696-704.

Research output: Contribution to journal › Article › Academic › peer-review

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T1 - A Longitudinal Evaluation of Partial Lung Irradiation in Mice by Using a Dedicated Image-Guided Small Animal Irradiator

AU - Granton, Patrick V.

AU - Dubois, Ludwig

AU - van Elmpt, Wouter

AU - van Hoof, Stefan J.

AU - Lieuwes, Natasja G.

AU - De Ruysscher, Dirk

AU - Verhaegen, Frank

PY - 2014/11/1

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N2 - Purpose: In lung cancer radiation therapy, the dose constraints are determined mostly by healthy lung toxicity. Preclinical microirradiators are a new tool to evaluate treatment strategies closer to clinical irradiation devices. In this study, we quantified local changes in lung density symptomatic of radiation-induced lung fibrosis (RILF) after partial lung irradiation in mice by using a precision image-guided small animal irradiator integrated with micro-computed tomography (CT) imaging. Methods and Materials: C57BL/6 adult male mice (n = 76) were divided into 6 groups: a control group (0 Gy) and groups irradiated with a single fraction of 4, 8, 12, 16, or 20 Gy using 5-mm circular parallel-opposed fields targeting the upper right lung. A Monte Carlo model of the small animal irradiator was used for dose calculations. Following irradiation, all mice were imaged at regular intervals over 39 weeks (10 time points total). Nonrigid deformation was used to register the initial micro-CT scan to all subsequent scans. Results: Significant differences could be observed between the 3 highest (>10 Gy) and 3 lowest irradiation (

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